Evidence map›Paper›PMID 42371582›Full record

ArticleBiophysics and physicobiology2026

Oligo DNA-based quantum dot (QD) single-particle tracking for multicolor single-molecule imaging.

Shigeo Sakuragi, Naoki Kato, Tomoya Uchida, Boxiao Zhao, Taro Katagiri, Miyu Enomoto, Rie Kato, Hideaki Yoshimura, Chisato Oyama, Iona Katayama and 3 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biophysics and physicobiology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Shigeo SakuragiDepartment of Electrical Engineering and Biosciences, School of Advanced Science and Engineering, Waseda University, Tokyo 162-0056, Japan.
Naoki KatoDepartment of Electrical Engineering and Biosciences, School of Advanced Science and Engineering, Waseda University, Tokyo 162-0056, Japan.
Tomoya UchidaDepartment of Electrical Engineering and Biosciences, School of Advanced Science and Engineering, Waseda University, Tokyo 162-0056, Japan.
Boxiao ZhaoDepartment of Electrical Engineering and Biosciences, School of Advanced Science and Engineering, Waseda University, Tokyo 162-0056, Japan.
Taro KatagiriDepartment of Electrical Engineering and Biosciences, School of Advanced Science and Engineering, Waseda University, Tokyo 162-0056, Japan.
Miyu EnomotoDepartment of Electrical Engineering and Biosciences, School of Advanced Science and Engineering, Waseda University, Tokyo 162-0056, Japan.
Rie KatoDepartment of Electrical Engineering and Biosciences, School of Advanced Science and Engineering, Waseda University, Tokyo 162-0056, Japan.
Hideaki YoshimuraDepartment of Chemistry, School of Science, The University of Tokyo, Tokyo 113-0033, Japan.
Chisato OyamaDepartment of Electrical Engineering and Biosciences, School of Advanced Science and Engineering, Waseda University, Tokyo 162-0056, Japan.
Iona KatayamaDepartment of Electrical Engineering and Biosciences, School of Advanced Science and Engineering, Waseda University, Tokyo 162-0056, Japan.
Ayano ChikumaDepartment of Electrical Engineering and Biosciences, School of Advanced Science and Engineering, Waseda University, Tokyo 162-0056, Japan.
Yuji TeramuraDepartment of Bioengineering, Graduate School of Engineering, The University of Tokyo, Tokyo 113-8656, Japan.
Hiroko BannaiDepartment of Electrical Engineering and Biosciences, School of Advanced Science and Engineering, Waseda University, Tokyo 162-0056, Japan.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Quantum dot single-particle tracking (QD-SPT), a powerful tool for analyzing membrane domains that are critical to various cellular processes, is widely used in membrane molecular dynamics research. QDs, which possess both a broad excitation range and a narrow emission bandwidth, are inherently suited for multicolor imaging at various wavelengths. However, applying multicolor QD-SPT with multiple biomolecular targets has been challenging due to the limited methods for specifically conjugating QDs to biomolecules. Here, we propose a DNA hybridization-based QD labeling method that generates several specific interactions based on sequence. QD fused with 20-mer oligo DNA specifically labeled membrane lipid 1,2-dipalmitoyl-sn-glycero-3-phosphatidylethanolamine (DPPE) covalently bound to complementary oligo DNA, forming a stable label that is suitable for SPT. The combination of polyA-polyT sequence as the linker oligo caused more QDs to fuse to DPPE compared with a random sequence linker. Oligo DNA-based QD-SPT accurately reflected the diffusion dynamics of DPPE measured using the single-fluorescence tracking technique and was compatible with conventional QD-SPT utilizing secondary antibody Fab. Using different pairs of oligo DNA sequences, we successfully achieved multicolor QD-SPT that distinguishes the lateral diffusion of DPPE and a membrane protein, GABA

Indexed as

diffusionmembrane moleculemulticolor imagingquantum dotsingle-particle tracking

Identifiers

PMID42371582
PMCPMC13310573

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.