ArticleInternational microbiology : the official journal of the Spanish Society for Microbiology2026
Sensitizing carbapenem-resistant Klebsiella pneumoniae using a compact Cascade-Cas3 system.
Article in International microbiology : the official journal of the Spanish Society for Microbiology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
backgroundKlebsiella pneumoniae is a 'superbug' characterized by high virulence and carbapenem resistance. CRISPR-Cas3 is capable of performing large-scale deletions in bacterial genomes. The purpose of this study is to sensitize Klebsiella pneumoniae A324 to carbapenem antibiotics by eliminating the multidrug resistance plasmid pA324-IMP using a compact Cascade-Cas3 system.
methodsRecombinant plasmids were constructed using molecular cloning techniques. Three sgRNAs targeting different positions within the bla
resultsIn this study, we successfully constructed the high copy number plasmid, the fluorescent plasmid, and the recombinant CRISPR plasmids. PCR and qPCR results demonstrated that the CRISPR-Cas3 system could efficiently eliminate bla
conclusionIn conclusion, this work demonstrates that the compact Cascade-Cas3 system can serve as a novel tool to eliminate the resistance gene bla
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