ArticleProtoplasma2026
Improving embryo rescue efficiency in seedless grape breeding with zeatin.
Article in Protoplasma, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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12 authors.
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Abstract
Embryo rescue is a crucial technique for developing new seedless grape germplasm; however, its current efficiency remains suboptimal. Optimizing the culture medium is essential for the success of embryo rescue, as it directly influences the development and germination of in vitro embryos. Therefore, this study systematically investigated the effects of parental genotypes and the addition of zeatin (ZT) to the culture medium on the efficiency of embryo rescue, using four self-pollinated maternal parents and 23 seedless×seeded (or seedless) cross combinations. The results showed that among the four self-pollinated parental lines, hybrid ovules exhibited the highest development rate in the ER medium, while a higher proportion of normal plantlets was obtained in the MM3 medium. Although the addition of ZT at various concentrations did not significantly enhance the embryo development rate in the self-pollinated maternal parents, it significantly improved germination outcomes: compared to the control, the rate of normal plantlet formation increased by 8.84-19.03%, while the proportions of abnormal plantlets and ungerminated embryos were significantly reduced. Further validation in hybrid combinations revealed that the addition of an appropriate concentration of ZT significantly increased both embryo development and plantlet formation rates in both MM3 and ER media. Specifically, in the MM3 medium supplemented with 0.5 mg L
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