ArticlePlanta2026
Cloning and functional verification of genes LjHD8 and LjMIXTA1 related to trichome development in Lonicera japonica Thunb.
Article in Planta, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors.
Funding
Abstract
The morphological characteristics and cellular development of plant trichomes have been extensively studied, but research on their developmental mechanisms in medicinal plants such as Lonicera japonica Thunb (L. japonica) remains limited. Glandular trichomes (GTs) serve as crucial sites for flavonoid synthesis in L. japonica, and are closely associated with medicinal material quality. This study conducted transcriptome analysis on the trichomes and trichomeless flower buds of L. japonica "Sijihua." Subsequently, two GT development-related genes, LjHD8 and LjMIXTA1, were screened through homologous alignment. They were cloned, and the properties and structures of the corresponding proteins were analyzed. We also examined the expression patterns of the two genes and determined GT density on flower buds at various developmental stages. Moreover, subcellular localization revealed that the LjHD8 and LjMIXTA1 proteins were located in the cell nucleus. The genetic transformation results of Nicotiana benthamiana (N. benth.) indicated that the GT density of the two transgenic N. benth. lines was significantly higher than that of the wild-type. Additionally, HPLC analysis showed that isorhamnetin-3-O-glucoside (iso-3-O-glu) in both transgenic N. benth. lines was higher than wild-type. Therefore, LjHD8 and LjMIXTA1 play important roles in GT development and flavonoid compound synthesis in L. japonica. These findings contribute to understanding the molecular mechanism of trichome development in L. japonica and provide a basis for the evaluation of medicinal material quality, while also offering an important theoretical foundation for breeding new varieties with high GT density.
Indexed as
Identifiers
42370951What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.