ArticleFrontiers in microbiology2026
The updated one-step multiplex RT-qPCR method for PRRSV classical strains, highly pathogenic strains and NADC30-like strains.
Article in Frontiers in microbiology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Introduction: PRRSV-2 prevalent strains mainly include C-PRRSV, HP-PRRSV, and NADC30-like, with the latter being the current dominant lineage. Due to the high recombination and genetic variation of PRRSV, existing RT-qPCR assays face an increasing risk of false negatives. Therefore, based on the current prevalent strain sequences, it is of great significance to update and establish a one-step multiplex RT-qPCR method that can simultaneously detect C-PRRSV, HP-PRRSV and NADC30-like. Methods: By downloading the latest prevalent strain full genome sequences from NCBI and isolating them in our laboratory, the conserved and type-specific target regions of the three strains were screened in the high-variable region of the Results: The established standard curve showed a good linear relationship within the range of 1 × 108 to 1 × 10 Discussion: This study successfully established a one-step multiplex RT-qPCR method based on current prevalent strain sequences, which offers high sensitivity, strong specificity, and good repeatability, and can be used for rapid differential diagnosis of the three PRRSV subtypes in clinical samples, thereby supporting precise diagnosis, epidemiological monitoring, and prevention and control of PRRSV in China.
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