ArticleFrontiers in microbiology2026
Pervasive mixed infections and recurrent begomovirus co-detection in symptomatic pumpkin plants from Guangxi, China.
Article in Frontiers in microbiology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Introduction: Pumpkin production in Guangxi, China, is increasingly affected by complex viral diseases, but the regional viral spectrum and mixed-infection structure in symptomatic pumpkin plants remain insufficiently characterized. Methods: We analyzed 289 symptomatic pumpkin samples collected across Guangxi during 2022-2025 using small-RNA sequencing for virus discovery and targeted PCR/RT-PCR assays for individual-sample confirmation. Virus prevalence, mixed-infection profiles, regional and host-associated differences, and virus-virus and symptom-virus associations were evaluated. Results: Small-RNA sequencing identified 15 candidate virus species, and targeted assays confirmed 11 viruses. Among the 289 samples, 284 (98.27%) were positive for at least one confirmed virus and 279 (96.54%) carried two or more viruses. The dataset was dominated by PuYmMV, SLCPHV, TLCCNV, ToLCNDV, and SLCCNV, which were detected in 87.89%, 87.89%, 83.39%, 78.89%, and 68.86% of samples, respectively. The most frequent infection profile was simultaneous detection of these five begomovirus species, occurring in 35.64% of samples. After Benjamini-Hochberg correction, ZTMV and PRSV were enriched in northern Guangxi, whereas TLCCNV and ToLCNDV were enriched in southern Guangxi. Host-species differences were not significant after correction. Pairwise analysis revealed a strong begomovirus-rich co-detection module, whereas symptom-virus associations were comparatively weak. Discussion: Symptomatic pumpkin viral disease in Guangxi is characterized by broad virus diversity, pervasive mixed infection, and recurrent begomovirus-rich co-detection. These findings provide a regional baseline for multiplex diagnosis, begomovirus-oriented surveillance, and future plant-vector-reservoir studies in pumpkin production systems.
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