Evidence map›Paper›PMID 42367981›Full record

ArticlebioRxiv : the preprint server for biology2026

Revealing interactions between glutathione peroxidase 4 and phosphoinositides.

Sara H Walters, ByungUk Park, Courtney L Labrecque, Faik N Musayev, Reid C Van Lehn, Brian Fuglestad

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Sara H WaltersDepartment of Chemistry, Virginia Commonwealth University, Richmond, Virginia, USA.
ByungUk ParkDepartment of Chemical and Biological Engineering, University of Wisconsin-Madison, Madison, Wisconsin 53706, USA.
Courtney L LabrecqueDepartment of Chemistry, Virginia Commonwealth University, Richmond, Virginia, USA.
Faik N MusayevDepartment of Medicinal Chemistry, School of Pharmacy, Virginia Commonwealth University, Richmond, Virginia 23298, USA.
Reid C Van LehnDepartment of Chemical and Biological Engineering, University of Wisconsin-Madison, Madison, Wisconsin 53706, USA.
Brian FuglestadDepartment of Chemistry, Virginia Commonwealth University, Richmond, Virginia, USA.

Funding

Peripheral membrane proteins and disease: tool development, basic investigations, and inhibitor designR35GM147221 · NIGMS · WISTAR INSTITUTE · PI Brian Fuglestad · 2022 to 2026
$2.1M
NIGMS NIH HHS R35 GM147221
6 · The paper itself

Abstract

Glutathione peroxidase 4 (GPx4) is the primary enzyme reducing lipid hydroperoxides, preventing membrane oxidative damage and protecting against ferroptosis. GPx4 is known to engage with lipid headgroups through electrostatic interactions, positioning the substrate for reduction. This work reveals and characterizes binding of highly anionic phosphoinositides (PIP lipids) by GPx4. PIPs are vital lipids in human cells and are central to many signaling processes, particularly in cytosolic facing membranes. Lipid overlay assays confirm interactions between GPx4 and phosphorylated PIPs, comparable to known anionic lipid binders. Protein NMR describes the interaction between GPx4 and PIPs within micelles. The greatest resonance shifting occurs with trisphosphorylated PIP, suggesting that higher anionic charge leads to greater binding, a known driver of GPx4 substrate recognition. Preferred anionic interactions were also confirmed with titration and crystallographic structure analysis of inositol phosphate 4 (IP

Indexed as

glutathione peroxidase 4peripheral membrane proteinphosphatidylinositol phosphateprotein crystallographyprotein NMR

Identifiers

PMID42367981
PMCPMC13307935

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.