Evidence map›Paper›PMID 42366733›Full record

ArticleBiotechnology and bioengineering2026

Metabolic Flux Analysis Reveals Cell Line-Specific Rewiring in CHO Cells Following TCA Cycle Intermediate Feeding for Bioprocess pH Control.

Sarah A Sacco, Kevin J Ruiz-Márquez, Rachel Moen, Irina Trenary, Xiaolin Zhang, Xiaowen Wang, Malik Padellan, Henry Lin, Jamey D Young

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biotechnology and bioengineering, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Sarah A SaccoDepartment of Chemical and Biomolecular Engineering, Vanderbilt University, Nashville, Tennessee, USA.ORCID https://orcid.org/0000-0001-8256-7884
Kevin J Ruiz-MárquezDepartment of Chemical and Biomolecular Engineering, Vanderbilt University, Nashville, Tennessee, USA.ORCID https://orcid.org/0000-0001-7546-2208
Rachel MoenDepartment of Chemical and Biomolecular Engineering, Vanderbilt University, Nashville, Tennessee, USA.ORCID https://orcid.org/0000-0001-7158-2106
Irina TrenaryDepartment of Chemical and Biomolecular Engineering, Vanderbilt University, Nashville, Tennessee, USA.
Xiaolin ZhangBiologics Process Research & Development, Process Research & Development, Merck & Co. Inc., Rahway, New Jersey, USA.
Xiaowen WangBiologics Process Research & Development, Process Research & Development, Merck & Co. Inc., Rahway, New Jersey, USA.ORCID https://orcid.org/0000-0003-3922-0126
Malik PadellanBiologics Process Research & Development, Process Research & Development, Merck & Co. Inc., Rahway, New Jersey, USA.ORCID https://orcid.org/0000-0003-1209-2806
Henry LinBiologics Process Research & Development, Process Research & Development, Merck & Co. Inc., Rahway, New Jersey, USA.ORCID https://orcid.org/0000-0002-8501-3980
Jamey D YoungDepartment of Chemical and Biomolecular Engineering, Vanderbilt University, Nashville, Tennessee, USA.ORCID https://orcid.org/0000-0002-0871-1494

Funding

Merck Sharp & Dohme LLC, a subsidiary of Merck & Co. Inc., Rahway, NJ, USA LKR194743)
6 · The paper itself

Abstract

Chinese hamster ovary (CHO) cells are the leading host for recombinant therapeutic protein production in the biopharma industry. In this study, we investigated how feeding acidic forms of tricarboxylic acid (TCA) cycle intermediates-malic acid, succinic acid, and α-ketoglutaric acid-affects cell culture performance and metabolism in two industrial IgG-producing CHO cell lines. These intermediates were used as pH control agents to replace conventional CO

Indexed as

Citric Acid CycleMetabolic Flux AnalysisAnimalsBioreactorsCHO CellsCricetinaeCricetulusCulture MediaHydrogen-Ion ConcentrationImmunoglobulin GKetoglutaric AcidsRecombinant ProteinsSuccinic AcidCulture MediaImmunoglobulin GKetoglutaric AcidsRecombinant ProteinsSuccinic Acid

Identifiers

PMID42366733
PMCPMC13576813

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.