Resistance-CONKAT-seq Guided Discovery of a ClpP Active Natural Product from a Soil Metagenome.

Jingbo Kan, Adrian Morales-Amador, Yozen Hernandez, Ján Burian, Melinda A Ternei, Sean F Brady

Abstract read

In one paragraph

Article in ACS chemical biology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

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0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

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5 · Who and what money

Authors and funding

6 authors.

Jingbo KanLaboratory of Genetically Encoded Small Molecules, The Rockefeller University, 1230 York Avenue, New York, New York 10065, United States.ORCID 0009-0006-1917-6577

Adrian Morales-AmadorLaboratory of Genetically Encoded Small Molecules, The Rockefeller University, 1230 York Avenue, New York, New York 10065, United States.ORCID 0000-0001-7944-308X

Yozen HernandezLaboratory of Genetically Encoded Small Molecules, The Rockefeller University, 1230 York Avenue, New York, New York 10065, United States.ORCID 0000-0003-3349-8856

Ján BurianLaboratory of Genetically Encoded Small Molecules, The Rockefeller University, 1230 York Avenue, New York, New York 10065, United States.

Melinda A TerneiLaboratory of Genetically Encoded Small Molecules, The Rockefeller University, 1230 York Avenue, New York, New York 10065, United States.

Sean F BradyLaboratory of Genetically Encoded Small Molecules, The Rockefeller University, 1230 York Avenue, New York, New York 10065, United States.ORCID 0000-0001-5967-8586

Funding

Discovery of Antibiotics from Soil Microbiomes Using MetagenomicsR35GM122559 · NIGMS · ROCKEFELLER UNIVERSITY · PI SEAN F BRADY · 2017 to 2026

$4.7M

NIGMS NIH HHS R35 GM122559

6 · The paper itself

Abstract

The discovery of natural products with specific molecular targets from metagenomes remains challenging. To address this limitation, we developed resistance-CONKAT-seq (resistance co-occurrence network analysis of targeted sequences) which links metagenomic BGCs (biosynthetic gene clusters) to potential modes of action through the identification of colocalized molecular target-based resistance genes. Applying this approach to a soil metagenomic library, we identified the uncharacterized

Indexed as

Bacterial ProteinsBiological ProductsEndopeptidase ClpMetagenomeSoil MicrobiologyMultigene FamilyMycobacterium tuberculosisBacterial ProteinsBiological ProductsEndopeptidase Clp

Identifiers

PMID42366525

DOI10.1021/acschembio.6c00347

PMCPMC13386464

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.