Expanded Allelic Diversity of Non-Classical HLA Class I and MIC Loci Identified Using Full-Gene Hybrid Capture-Based Next-Generation Sequencing.

Itta Krishna Chaaithanya, Pawan Kumar Raghav, Nancy Lee, Carla Wirtz, Denice Kong, Raja Rajalingam

Abstract read

In one paragraph

Article in HLA, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Itta Krishna ChaaithanyaImmunogenetics and Transplantation Laboratory (ITL), University of California, San Francisco (UCSF), San Francisco, California, USA.ORCID https://orcid.org/0000-0003-0702-2782

Pawan Kumar RaghavImmunogenetics and Transplantation Laboratory (ITL), University of California, San Francisco (UCSF), San Francisco, California, USA.ORCID https://orcid.org/0000-0002-2440-7134

Nancy LeeImmunogenetics and Transplantation Laboratory (ITL), University of California, San Francisco (UCSF), San Francisco, California, USA.ORCID https://orcid.org/0000-0003-3044-9522

Carla WirtzCareDx, West Chester, Pennsylvania, USA.ORCID https://orcid.org/0009-0000-3795-6127

Denice KongImmunogenetics and Transplantation Laboratory (ITL), University of California, San Francisco (UCSF), San Francisco, California, USA.ORCID https://orcid.org/0009-0009-7613-6629

Raja RajalingamImmunogenetics and Transplantation Laboratory (ITL), University of California, San Francisco (UCSF), San Francisco, California, USA.ORCID https://orcid.org/0000-0001-8821-7877

Funding

ICMR-DHR International Fellowship for Young Indian Biomedical Scientists

6 · The paper itself

Abstract

Non-classical HLA Class I genes (HLA-E, -F, -G, -H) and MHC-related genes (MICA, MICB) regulate NK cell function and immune tolerance, yet their allelic diversity remains underexplored. Using capture-enriched full-gene sequencing of 943 healthy US transplant donors, we identified 26 novel alleles across HLA-E (2), HLA-F (6), HLA-G (4), HLA-H (5), MICA (3), and MICB (6). Each novel allele was identified in a single individual and differs from its closest known allele by a single-nucleotide polymorphism within the coding region. In addition, we confirmed the recently described allele HLA-G*01:01:33 in 16 unrelated donors. In all cases, it consistently co-segregated with HLA-A*34:01:01, HLA-F*01:01:01 and HLA-H*02:27, supporting the presence of a conserved haplotype. These findings expand the catalogue of non-classical HLA and MIC alleles, highlighting capture-enriched full-gene sequencing as a powerful tool for comprehensive immunogenetic profiling.

Indexed as

AllelesHigh-Throughput Nucleotide SequencingHistocompatibility Antigens Class IPolymorphism, Single NucleotideHaplotypesHistocompatibility TestingHLA-E AntigensHumansSequence Analysis, DNATissue DonorsHistocompatibility Antigens Class IHLA-E AntigensMHC class I-related chain AMICB antigenfull‐gene HLA sequencingHLA‐EHLA‐FHLA‐GHLA‐Hhybrid capture–based NGSMICA and MICBnon‐classical HLA Class Inovel HLA alleles

Identifiers

PMID42366455

DOI10.1111/tan.70824

PMCPMC13310964

What OpenQuestion holds

Textmetadata

LicenceCC BY

Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.