Evidence map›Paper›PMID 42365554›Full record

ArticleBiodegradation2026

Enhancing chlorpyrifos degradation and plant growth through biochar-assisted microbial remediation.

M Kiran, R Sindhu, M Supreeth

Abstract read
In one paragraph

Article in Biodegradation, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

M KiranDepartment of Microbiology, JSS Academy of Higher Education and Research, Mysuru, Karnataka, 570015, India.
R SindhuDepartment of Microbiology, JSS Academy of Higher Education and Research, Mysuru, Karnataka, 570015, India.
M SupreethDepartment of Microbiology, JSS Academy of Higher Education and Research, Mysuru, Karnataka, 570015, India. supreeth@jssuni.edu.in.

Funding

JSS AHER Research Scholar Seed Money grant JSSAHER/REG/RES/URG/54/2025-26 Dated:08.04.2026
6 · The paper itself

Abstract

The extensive use of chlorpyrifos (CPF) in agriculture has led to persistent soil contamination, posing serious risks to environmental and human health. Developing sustainable, in situ remediation strategies is therefore a priority. This study evaluated an integrated plant-microbe-biochar system for the remediation of CPF-contaminated soils. Indigenous CPF-degrading bacteria were isolated from agricultural soils and screened for degradation efficiency in mineral salts medium (MSM) and soil microcosms. The most efficient isolate, designated C78, was identified by 16S rRNA gene sequencing as Klebsiella pneumoniae. In MSM supplemented with 200 mg/L CPF, K. pneumoniae C78 degraded > 80% of the pesticide within 72 h. The strain also exhibited multiple plant growth-promoting traits, including indole-3-acetic acid production, phosphate solubilization, and ammonia production, and retained these functions in the presence of CPF. Soil microcosm and pot experiments were conducted using Ricinus communis L. grown in soils spiked with CPF (100 and 200 mg/kg), with and without biochar amendment and bacterial inoculation. Inoculation with K. pneumoniae C78 significantly accelerated CPF dissipation compared with non-inoculated controls, and the combined treatment with biochar further enhanced degradation, leading to near-complete CPF removal within 21 days. GC-MS/MS analysis showed no accumulation of common toxic intermediates such as 3,5,6-trichloro-2-pyridinol (TCP) or DETP/TMP. Biochar amendment improved soil conditions and supported higher microbial activity, while plant-microbe interactions in the rhizosphere promoted seed germination, seedling vigor, biomass production, chlorophyll content, and protein levels under CPF stress. Overall, the results demonstrate that integrating CPF-degrading K. pneumoniae C78 with R. communis L. and biochar amendment constitutes a promising, eco-compatible strategy for enhancing CPF degradation and restoring the productivity of contaminated agricultural soils.

Indexed as

CharcoalChlorpyrifosSoil PollutantsBiodegradation, EnvironmentalInsecticidesPlant DevelopmentRNA, Ribosomal, 16SSoil MicrobiologybiocharCharcoalChlorpyrifosInsecticidesRNA, Ribosomal, 16SSoil PollutantsBiochar amendmentBioremediationChlorpyrifosPlant-microbe interactionsSoil contamination

Identifiers

PMID42365554
PMCPMC13310836

What OpenQuestion holds

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LicenceCC BY-NC-ND
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.