Thyroxin displacement from single and combined thyroid hormone binding proteins by various test substances.

J Brandt, E Fabian, A Aktalay-Hippchen, L Jedermann, R Landsiedel

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Article in Archives of toxicology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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5 · Who and what money

Authors and funding

5 authors.

J BrandtPharmacy, Pharmacology and Toxicology, Free University of Berlin, Berlin, Germany.

E FabianBASF SE, Experimental Toxicology and Ecology, Ludwigshafen am Rhein, Germany.

A Aktalay-HippchenBASF SE, Experimental Toxicology and Ecology, Ludwigshafen am Rhein, Germany.

L JedermannBASF SE, Experimental Toxicology and Ecology, Ludwigshafen am Rhein, Germany.

R LandsiedelPharmacy, Pharmacology and Toxicology, Free University of Berlin, Berlin, Germany. robert.landsiedel@fu-berlin.de.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The thyroid hormone system regulates physiological processes that are crucial for growth and metabolism. The homeostasis of thyroid hormones can be affected by test substances via multiple molecular interactions, one of them being the displacement of thyroid hormones from their binding proteins. The main thyroid hormone in the human bloodstream is L-thyroxine (T4), ≥ 99.5% of which is transported bound to three proteins: thyroxine binding globulin (TBG), transthyretin (TTR) and human serum albumin (HSA). To investigate the interactions of xenobiotics with the binding of T4 to these proteins, an in vitro thyroid hormone protein binding assay (THPB-assay) was developed. Samples containing 100 nM T4 were incubated with a T4-binding protein and increasing concentrations of a test compound, followed by removal of the unbound T4 via size exclusion chromatography and analysis of protein-bound T4. This assay allows for multiple adjustable parameters, including the choice of binding protein and the detection method for protein-bound T4, which can be extracted and measured via LC-MS or, when using

Indexed as

Endocrine disruptionKey eventSerum protein bindingThyroid hormone homeostasisThyroxine (T4) displacement

Identifiers

PMID42365110

DOI10.1210/endo-116-4-1248

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.