Update of Mexican population databases for complex kinship interpretation with twelve and seven X-STRs.

backgroundX-chromosome short tandem repeats (X-STRs) are valuable for resolving complex kinship cases. Most population databases are based on the ArgusX12 kit (Qiagen), using PCR and capillary electrophoresis (CE) to identify length-based (LB) alleles, and are formatted for FamlinkX software. However, haplotype diversity is often underrepresented by population studies, compelling database updates ( https://famlink.se/fx_databases.html ). The ForenSeq DNA Signature Prep kit (Verogen) uses massive parallel sequencing (MPS) to analyze 7 X-STRs and report sequence-based (SB) alleles, for which population databases are currently unavailable. Because these 7 markers are a subset of the ArgusX12 panel, existing LB-based databases can support kinship interpretation of MPS-derived data. METHODS AND

resultsWe therefore updated a Mexican population database for 12 X-STRs and generated a corresponding 7-marker subset with FamlinkX-compatible files. Using 500 haplotypes from the Investigator Argus X-12 kit QS (Qiagen), we estimated forensic parameters and evaluated the impact of the updated database by calculating likelihood ratios (LRs) in four representative kinship scenarios. The updated database (n = 1433) increased LRs by an average of 55% compared to the previous version (n = 933).

conclusionsThese results support improved forensic interpretation in Mexican and Latin American populations lacking comprehensive national X-STR databases based on ArgusX12 and ForenSeq DNA Signature kits.