ArticleNpj viruses2026
Single molecule super resolution microscopy reveals formation of Dengue NS2B3 cluster on mitochondrial network and its effect on fragmentation.
Article in Npj viruses, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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4 authors.
Funding
Abstract
It is challenging to understand viral proteins at a single-molecule level and visualize their dynamics in a cellular environment. This calls for sophisticated microscopy techniques (such as,SMLM) and single-molecule analysis that can reveal details with the precision of a single molecule. In Dengue type-2(Denv - 2) infection, NS2B3 is recognized as a critical protein complex responsible for proteolytic activity and processing of viral polyprotein. We study the distribution of NS2B3 protein and its clusters. Accordingly, two distinct photoactivatable probes(mEos - NS2B3 and PAGFP - NS2B3) and a fluorescent probe (eGFP-NS2B3) were constructed. Results confirmed the formation of NS2B3 clusters in close vicinity to the mitochondrial network. Statistical analysis of single-molecule data (images) facilitated the estimation of critical cluster parameters(such as cluster density, number of molecules/cluster,and its spread). In addition, regional analysis suggests a correlation between NS2B3 cluster formation and fragmentation of mitochondrial network which is further supported by time-lapse confocal imaging. In other words, the presence of cluster depletes mitochondrial segment. To further demonstrate, time-lapse super-resolution imaging is carried out that enabled visualization of the formation of NS2B3 clusters. Understanding the underlying dynamics of NS2B3 clustering at the single-molecule level may facilitate the identification of potential drug targets,and methods to disrupt them.
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