Protocol for live cell barcoding and immunophenotyping of human hematological malignancies using cytometry by time of flight.

Takashi Mikami, James Badger Wing, Junko Takita, Itaru Kato

Abstract read

In one paragraph

Article in STAR protocols, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Takashi MikamiDivision of Cancer Immune Multicellular System Regulation, Center for Cancer Immunotherapy and Immunobiology (CCII), Graduate School of Medicine, Kyoto University, Kyoto 606-8501, Japan; Department of Pediatrics, Graduate School of Medicine, Kyoto University, Kyoto 606-8507, Japan. Electronic address: mikami.takashi.3r@kyoto-u.ac.jp.

James Badger WingLaboratory of Human Single Cell Immunology, Immunology Frontier Research Center (IFReC), The University of Osaka, Suita, Osaka 565-0871, Japan; Human Single Cell Immunology Team, Center for Infectious Disease Education and Research (CiDER), The University of Osaka, Suita, Osaka 565-0871, Japan; Center for Advanced Modalities and DDS (CAMaD), The University of Osaka, Suita, Osaka 565-0871, Japan.

Junko TakitaDepartment of Pediatrics, Graduate School of Medicine, Kyoto University, Kyoto 606-8507, Japan.

Itaru KatoDepartment of Pediatrics, Graduate School of Medicine, Kyoto University, Kyoto 606-8507, Japan. Electronic address: itarkt@kuhp.kyoto-u.ac.jp.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

High-dimensional cell profiling by cytometry by time of flight (CyTOF) enables simultaneous characterization of rare cell populations but requires many antibodies and is sensitive to staining variability. Here, we present a workflow for immunophenotyping human hematological malignancy samples, reducing costs and minimizing technical variation. We describe steps for sample collection, live-cell barcoding using anti-β2-microglobulin and CD147 antibodies, cell staining, data collection, and subsequent analyses. For complete details on the use and execution of this protocol, please refer to Mikami et al.

Indexed as

CancerImmunologyMass Cytometry

Identifiers

PMID42360882

DOI10.1016/j.xpro.2026.104663

PMCPMC13333325

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LicenceCC BY-NC-ND

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.