ArticleTropical animal health and production2026
Development of recombinant glycoprotein B based Indirect ELISA for detection of antibodies against Bovine alphaherpesvirus-1 in Bovines.
Article in Tropical animal health and production, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Infectious Bovine Rhinotracheitis (IBR) is a highly contagious respiratory disease of cattle caused by Bovine alphaherpesvirus-1 (BoAHV-1), a member of the genus Varicellovirus. Similar to other alphaherpesviruses, BoAHV-1 establishes lifelong latency with the potential for reactivation under immunosuppressive or stressful conditions. The viral envelope contains several glycoproteins, among which gB, gC, and gD are the major immunogens recognized by sera of infected cattle. Glycoprotein B (gB) is highly conserved and strongly immunogenic, making it a suitable target for diagnostic assay development. In this study, a truncated gB fragment was cloned and expressed in Escherichia coli. The recombinant gB (rgB) (~ 47 kDa) was purified under denaturing conditions using Ni-NTA affinity chromatography and confirmed by SDS-PAGE and western blotting. The protein showed strong reactivity with anti-His antibodies and IBR-positive bovine sera, indicating preserved immunoreactive epitopes. The purified protein was standardized as a coating antigen in an indirect ELISA (iELISA). Diagnostic evaluation using 162 sera, compared with the ID Screen
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