ArticleFrontiers in plant science2026
An optimized, rhamnolipid-containing cell-free filtrate from
Article in Frontiers in plant science, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Introduction: The biocontrol strain Methods: Fermentation was optimized through medium screening, single-factor experiments, and orthogonal array design. The resulting cell‑free filtrate (CFF) was evaluated for antifungal activity against 19 tropical crop pathogens, environmental stability (thermal, pH, UV, reductants, metal ions), rhamnolipid composition (TLC, HPLC‑MS, ¹H NMR), field efficacy against rubber tree powdery mildew ( Results: The optimal medium (15 g/L acid‑hydrolyzed casein, 25 mL/L glycerol, 1 g/L MgSO₄·7H₂O, 1.5 g/L K₂HPO₄, pH 6.5) and culture conditions (50 mL/500 mL flask, 220 rpm, 10% inoculum, 36 °C, 5 days) increased antifungal activity against Conclusion: The optimized CFF combines broad‑spectrum activity, exceptional stability, and field efficacy with biosafety, making it a promising low‑cost biocontrol candidate for sustainable tropical agriculture.
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