Evidence map›Paper›PMID 42358726›Full record

ArticleJACS Au2026

Charting the Biosynthetic Landscape of Hybrid Polyketide-Nonribosomal Peptide-Specialized Lipids.

Fatima El Arnouki Belhaji, Dries De Ruysscher, Giel Vanreppelen, Laura-Lynn Huybrechts, Mohammad M Alanjary, Mitja M Zdouc, Emmanuel L C de Los Santos, Joachim Demaerel, Ewoud Vaneeckhaute, Odessa Van Goethem and 7 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in JACS Au, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

17 authors.

Fatima El Arnouki BelhajiLaboratory for Biomolecular Discovery and Engineering, Department of Biology, KU Leuven, 3001 Leuven, Belgium.
Dries De RuysscherLaboratory for Biomolecular Discovery and Engineering, Department of Biology, KU Leuven, 3001 Leuven, Belgium.
Giel VanreppelenLaboratory for Biomolecular Discovery and Engineering, Department of Biology, KU Leuven, 3001 Leuven, Belgium.ORCID https://orcid.org/0000-0002-0791-2446
Laura-Lynn HuybrechtsLaboratory for Biomolecular Discovery and Engineering, Department of Biology, KU Leuven, 3001 Leuven, Belgium.
Mohammad M AlanjaryBioinformatics Group, Wageningen University & Research, 6708 PB Wageningen, The Netherlands.
Mitja M ZdoucBioinformatics Group, Wageningen University & Research, 6708 PB Wageningen, The Netherlands.
Emmanuel L C de Los SantosDepartment of Chemistry, University of Warwick, CV4 7AL Coventry, U.K.
Joachim DemaerelLaboratory of Organic Synthesis, Department of Chemistry, KU Leuven, 3001 Leuven, Belgium.ORCID https://orcid.org/0000-0003-3685-7535
Ewoud VaneeckhauteNMR/X-ray platform for Convergence Research (NMRCoRe), Centre for Surface Chemistry and Catalysis - Characterization and Application Team (COK-KAT), KU Leuven, 3001 Leuven, Belgium.
Odessa Van GoethemLaboratory of Molecular Cell Biology, Department of Biology, KU Leuven, 3001 Leuven, Belgium.
Hans GerstmansLaboratory for Biomolecular Discovery and Engineering, Department of Biology, KU Leuven, 3001 Leuven, Belgium.ORCID https://orcid.org/0000-0002-7469-5146
Eric BreynaertNMR/X-ray platform for Convergence Research (NMRCoRe), Centre for Surface Chemistry and Catalysis - Characterization and Application Team (COK-KAT), KU Leuven, 3001 Leuven, Belgium.ORCID https://orcid.org/0000-0003-3499-0455
Patrick Van DijckLaboratory of Molecular Cell Biology, Department of Biology, KU Leuven, 3001 Leuven, Belgium.
Marnix H MedemaBioinformatics Group, Wageningen University & Research, 6708 PB Wageningen, The Netherlands.ORCID https://orcid.org/0000-0002-2191-2821
Angus N M WeirLaboratory for Biomolecular Discovery and Engineering, Department of Biology, KU Leuven, 3001 Leuven, Belgium.
Eveline LescrinierLaboratory for Medicinal Chemistry, Department of Pharmaceutical and Pharmacological Sciences, Rega Institute for Medical Research, KU Leuven, 3001 Leuven, Belgium.ORCID https://orcid.org/0000-0001-7066-4329
Joleen MasscheleinLaboratory for Biomolecular Discovery and Engineering, Department of Biology, KU Leuven, 3001 Leuven, Belgium.ORCID https://orcid.org/0000-0003-4366-3675

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Polyunsaturated fatty acid (PUFA) synthase enzymes are best known for their role in membrane lipid biosynthesis in marine psychrophilic bacteria but have also evolved to assemble specialized lipid-containing metabolites with unique biological functions. Here, we illuminate their broader biosynthetic potential by charting the unexplored landscape of hybrid peptide-polyketide-specialized lipid biosynthesis in bacteria. Using a targeted genome mining strategy, we identified more than 60 biosynthetic gene clusters that encode PUFA synthase-like, polyketide synthase (PKS), and nonribosomal peptide synthetase (NRPS) enzymes across diverse bacterial lineages. Comparative analysis revealed extensive diversification of these triple hybrid pathways through gene fusion, domain reshuffling, and enzyme recruitment. We further expand the known repertoire of peptide-polyketide-specialized lipid hybrids by identifying the chitinimines, a new family of amphiphilic metabolites produced by

Indexed as

genome miningnatural productsnonribosomal peptidespolyketidespolyunsaturated fatty acids

Identifiers

PMID42358726
PMCPMC13291983

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.