Evidence map›Paper›PMID 42358703›Full record

ArticleJACS Au2026

Functional DNAzyme-Guided Target Identification and Portable Detection of Colorectal Cancer.

Yu Yan, Hao Zheng, Shenyang Zhao, Lishuang Lin, Qinqin Hu, Jie Liu, Hongzhou Gu

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in JACS Au, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Yu YanState Key Laboratory of Synergistic Chem-Bio Synthesis, School of Chemistry and Chemical Engineering, Frontiers Science Center for Transformative Molecules, the International Peace Maternity and Child Health Hospital, School of Medicine, National Center for Translational Medicine, Shanghai Jiao Tong University, Shanghai 200030, China.
Hao ZhengState Key Laboratory of Synergistic Chem-Bio Synthesis, School of Chemistry and Chemical Engineering, Frontiers Science Center for Transformative Molecules, the International Peace Maternity and Child Health Hospital, School of Medicine, National Center for Translational Medicine, Shanghai Jiao Tong University, Shanghai 200030, China.
Shenyang ZhaoDepartment of Digestive Diseases, National Clinical Research Center for Aging and Medicine, Huashan Hospital, Fudan University, Shanghai 200040, China.
Lishuang LinDepartment of Digestive Diseases, National Clinical Research Center for Aging and Medicine, Huashan Hospital, Fudan University, Shanghai 200040, China.
Qinqin HuState Key Laboratory of Synergistic Chem-Bio Synthesis, School of Chemistry and Chemical Engineering, Frontiers Science Center for Transformative Molecules, the International Peace Maternity and Child Health Hospital, School of Medicine, National Center for Translational Medicine, Shanghai Jiao Tong University, Shanghai 200030, China.ORCID https://orcid.org/0000-0001-5726-9279
Jie LiuDepartment of Digestive Diseases, National Clinical Research Center for Aging and Medicine, Huashan Hospital, Fudan University, Shanghai 200040, China.ORCID https://orcid.org/0000-0002-7638-187X
Hongzhou GuState Key Laboratory of Synergistic Chem-Bio Synthesis, School of Chemistry and Chemical Engineering, Frontiers Science Center for Transformative Molecules, the International Peace Maternity and Child Health Hospital, School of Medicine, National Center for Translational Medicine, Shanghai Jiao Tong University, Shanghai 200030, China.ORCID https://orcid.org/0000-0001-5058-4815

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Rapid discrimination of colorectal cancer (CRC) at the time of tissue sampling remains clinically challenging. Although DNAzyme-based assays enable selective analysis of complex biological samples, their diagnostic translation is constrained by undefined endogenous activators. Here, using an iterative DNAzyme activity-guided isolation strategy that integrates chromatographic fractionation with affinity enrichment, we identified malic enzyme 1 (ME1) as the molecular activator of a DNAzyme newly selected against colorectal cancer tissue lysates. The ME1-triggered catalytic reaction was then translated into a lateral flow format, enabling instrument-free visual detection within 35 min. In clinical specimens, the platform discriminated CRC from low- and high-grade intraepithelial neoplasia with an AUC of 0.98. By defining the molecular basis of DNAzyme activation and coupling it to a portable readout, this work provides a direct route from evolving DNAzyme probes for sensing complex tissues to rapid, near-point-of-care detection of CRC.

Indexed as

colorectal cancerDNAzymemalic enzyme 1point-of-care diagnosticstarget identification

Identifiers

PMID42358703
PMCPMC13291997

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.