Evidence map›Paper›PMID 42357754›Full record

ArticleVeterinary sciences2026

Establishment and Characterization of an Immortalized Porcine Satellite Cell Line from China Junmu No.1 Pigs.

Jing Li, Yu He, Xiaoran Zhang, Jiayi Ning, Dali Wang, Chunyan Bai, Boxing Sun, Shaoxuan Zhang, Shuang Liang, Hao Sun

Abstract read
In one paragraph

Article in Veterinary sciences, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Jing LiDepartment of Animal Sciences, College of Animal Sciences, Jilin University, Changchun 130062, China.
Yu HeDepartment of Animal Sciences, College of Animal Sciences, Jilin University, Changchun 130062, China.
Xiaoran ZhangDepartment of Animal Sciences, College of Animal Sciences, Jilin University, Changchun 130062, China.
Jiayi NingDepartment of Animal Sciences, College of Animal Sciences, Jilin University, Changchun 130062, China.
Dali WangDepartment of Animal Sciences, College of Animal Sciences, Jilin University, Changchun 130062, China.
Chunyan BaiDepartment of Animal Sciences, College of Animal Sciences, Jilin University, Changchun 130062, China.ORCID 0000-0002-4271-1893
Boxing SunDepartment of Animal Sciences, College of Animal Sciences, Jilin University, Changchun 130062, China.
Shaoxuan ZhangDepartment of Animal Sciences, College of Animal Sciences, Jilin University, Changchun 130062, China.
Shuang LiangDepartment of Animal Sciences, College of Animal Sciences, Jilin University, Changchun 130062, China.ORCID 0000-0003-2063-1662
Hao SunDepartment of Animal Sciences, College of Animal Sciences, Jilin University, Changchun 130062, China.

Funding

Jilin University 2024CX151National Natural Science Foundation of China 32202628
6 · The paper itself

Abstract

Junmu No.1 is a commercially important Chinese pig breed, yet stable in vitro models for investigating its muscle development mechanisms and genetic regulation remain lacking; this study aimed to establish an immortalized porcine satellite cell line from Junmu No.1 pigs to address this gap. Primary porcine satellite cells (PSCs) were isolated from a 2-day-old Junmu No.1 piglet and immortalized via lentiviral transduction using the pHAGE-EF1α-eGFP-SV40LT-BleoR vector. The resulting cell line (imPSC-JM) was characterized for morphology, satellite cell marker expression, karyotype stability, myogenic differentiation capacity, and long-term proliferative potential, and RNA sequencing combined with Gene Set Enrichment Analysis (GSEA) was performed to assess transcriptomic fidelity relative to primary PSCs. The imPSC-JM line retained characteristic spindle-shaped satellite cell morphology, consistently expressed PAX7, maintained a normal diploid karyotype (2

Indexed as

immortalized porcine satellite cellsJunmu No.1 pigsmuscle developmentmyogenic differentiationSV40 large T antigentranscriptomic analysis

Identifiers

PMID42357754
PMCPMC13308346

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.