Evidence map›Paper›PMID 42357753›Full record

ArticleVeterinary sciences2026

Porcine Erythrocyte-PRRSV Interactions: Implications for Targeted Nanodrug Delivery.

Wei Yin, Jingze Li, Haoxiang Yao, Jingyi Qiao, Jia Zhong, Yaogui Sun, Hongquan Li, Kuohai Fan, Zhenbiao Zhang, Na Sun and 3 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Veterinary sciences, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
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0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Wei YinShanxi Key Laboratory for Modernization of TCVM, College of Veterinary Medicine, Shanxi Agricultural University, Jinzhong 030801, China.
Jingze LiShanxi Key Laboratory for Modernization of TCVM, College of Veterinary Medicine, Shanxi Agricultural University, Jinzhong 030801, China.
Haoxiang YaoShanxi Key Laboratory for Modernization of TCVM, College of Veterinary Medicine, Shanxi Agricultural University, Jinzhong 030801, China.
Jingyi QiaoShanxi Key Laboratory for Modernization of TCVM, College of Veterinary Medicine, Shanxi Agricultural University, Jinzhong 030801, China.
Jia ZhongShanxi Key Laboratory for Modernization of TCVM, College of Veterinary Medicine, Shanxi Agricultural University, Jinzhong 030801, China.
Yaogui SunShanxi Key Laboratory for Modernization of TCVM, College of Veterinary Medicine, Shanxi Agricultural University, Jinzhong 030801, China.
Hongquan LiShanxi Key Laboratory for Modernization of TCVM, College of Veterinary Medicine, Shanxi Agricultural University, Jinzhong 030801, China.
Kuohai FanShanxi Key Laboratory for Modernization of TCVM, College of Veterinary Medicine, Shanxi Agricultural University, Jinzhong 030801, China.
Zhenbiao ZhangShanxi Key Laboratory for Modernization of TCVM, College of Veterinary Medicine, Shanxi Agricultural University, Jinzhong 030801, China.
Na SunShanxi Key Laboratory for Modernization of TCVM, College of Veterinary Medicine, Shanxi Agricultural University, Jinzhong 030801, China.
Panpan SunShanxi Key Laboratory for Modernization of TCVM, College of Veterinary Medicine, Shanxi Agricultural University, Jinzhong 030801, China.
Huizhen YangShanxi Key Laboratory for Modernization of TCVM, College of Veterinary Medicine, Shanxi Agricultural University, Jinzhong 030801, China.
Jianzhong WangShanxi Key Laboratory for Modernization of TCVM, College of Veterinary Medicine, Shanxi Agricultural University, Jinzhong 030801, China.ORCID 0000-0002-3349-2318

Funding

Science and Technology Department of Shanxi Province 20210302123407
6 · The paper itself

Abstract

objectiveTo establish a porcine complement receptor type 1-like (CR1-like)-mediated targeted anti-porcine reproductive and respiratory syndrome virus (PRRSV) nanodrug delivery system by investigating interactions between erythrocytes from Landrace piglets (both male and female), PRRSV, and anti-PRRSV nanodrugs.

methodsOptimal conditions for PRRSV sensitization with fresh porcine serum were determined. CR1-like-dependent immune adhesion of porcine erythrocytes to sensitized PRRSV was verified by immunofluorescence, electron microscopy, qPCR, and Western blot. The effect of this adhesion on PRRSV infection of porcine alveolar macrophages (PAMs) was studied using a flow chamber system. Mannose-modified matrine nanoliposomes (MMLNPs) were prepared, characterized, and evaluated for cytotoxicity, targeting ability, and in vitro antiviral activity.

resultsPRRSV was optimally sensitized by incubation with fresh porcine serum at 37 °C for 2 h. Porcine erythrocytes specifically adhered to sensitized PRRSV via CR1-like, significantly promoting PRRSV infection of PAMs. Stable, uniform-sized MMLNPs showed no cytotoxicity, targeted PAMs via CR1-like, and exhibited superior antiviral activity to free matrine.

conclusionsCR1-like-mediated immune adhesion is a critical mechanism for PRRSV infection of PAMs. Harnessing this natural pathway enables efficient targeted delivery of matrine nanoliposomes to PAMs, providing a promising translational strategy for PRRSV control.

Indexed as

mannosematrinePAMsporcine CR1-likePRRSV

Identifiers

PMID42357753
PMCPMC13307653

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