ArticleVeterinary sciences2026
Rapid Detection of Chicken Infectious Anemia Virus Using a One-Tube RPA-CRISPR/Cas12a System.
Article in Veterinary sciences, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Chicken infectious anemia virus (CIAV) is a major immunosuppressive pathogen that causes significant economic losses to the global poultry industry. Conventional detection methods for CIAV are limited by poor timeliness, high equipment requirements, and insufficient sensitivity. To address these challenges, this study developed a novel one-tube integrated RPA-CRISPR/Cas12a assay targeting the highly conserved VP3 gene of CIAV for rapid and accurate detection. The performance of the assay was comprehensively evaluated in terms of sensitivity, specificity, and repeatability. Its clinical utility was assessed by testing 80 clinical suspected samples, with quantitative real-time PCR (qPCR) serving as the reference method. The results showed that the limit of detection (LoD) of the developed method was 10 copies/reaction, comparable to that of qPCR. No cross-reactivity with common avian pathogens was observed. The intra- and inter-assay coefficients of variation (CV%) for the time to threshold (Tt) were both below 10%. In clinical sample detection, the assay achieved a total coincidence rate of 97.5%, with a sensitivity of 100% and specificity of 96% relative to qPCR. In conclusion, the RPA-CRISPR/Cas12a assay developed in this study offers rapid detection, high sensitivity and specificity, operational simplicity, low equipment dependency, and excellent repeatability. It provides a practical tool for early and rapid diagnosis of CIAV, clinical sample screening in grassroots veterinary laboratories, and on-site epidemiological surveillance in poultry farms, holding significant potential for the precise prevention, control, and eradication of CIAV in the poultry industry.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.