Evidence map›Paper›PMID 42357703›Full record

ArticleVeterinary sciences2026

BVDV NS5A Binds to CKAP2 and Activates the PI3K/AKT/mTOR Pathway to Facilitate Virus Transmission Through Tunneling Nanotubes.

Jiying Yin, Yanan Zhu, Jiating Zhang, Zehui Zhou, Ning He, Hongming Zhou, Xiaoqun Liu, Yixing Zhao, Longge Zhao, Ying Zong and 4 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Veterinary sciences, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors.

Jiying YinCollege of Animal Science and Technology, Jilin Agricultural University, Changchun 130118, China.
Yanan ZhuCollege of Animal Science and Technology, Jilin Agricultural University, Changchun 130118, China.
Jiating ZhangCollege of Animal Science and Technology, Jilin Agricultural University, Changchun 130118, China.
Zehui ZhouCollege of Animal Science and Technology, Jilin Agricultural University, Changchun 130118, China.
Ning HeCollege of Animal Science and Technology, Jilin Agricultural University, Changchun 130118, China.
Hongming ZhouCollege of Animal Science and Technology, Jilin Agricultural University, Changchun 130118, China.
Xiaoqun LiuCollege of Animal Science and Technology, Jilin Agricultural University, Changchun 130118, China.
Yixing ZhaoCollege of Animal Science and Technology, Jilin Agricultural University, Changchun 130118, China.
Longge ZhaoCollege of Animal Science and Technology, Jilin Agricultural University, Changchun 130118, China.
Ying ZongCollege of Chinese Medicinal Materials, Jilin Agricultural University, Changchun 130118, China.ORCID 0000-0003-0730-0519
Naichao DiaoCollege of Chinese Medicinal Materials, Jilin Agricultural University, Changchun 130118, China.
Kun ShiCollege of Chinese Medicinal Materials, Jilin Agricultural University, Changchun 130118, China.
Nan LiInstitute of Animal Husbandry and Veterinary Medicine, Changchun Academy of Agricultural Sciences, Changchun 130062, China.
Rui DuCollege of Chinese Medicinal Materials, Jilin Agricultural University, Changchun 130118, China.

Funding

Jilin Provincial Natural Science Foundation of China grant number 20230101255JC
6 · The paper itself

Abstract

Bovine viral diarrhea virus (BVDV), a significant global pathogen threatening cattle industries worldwide, presents substantial challenges for disease control. Its ability to infect cattle across all age groups, coupled with incompletely understood transmission mechanisms, complicates prevention and treatment strategies. We previously reported that BVDV induced tunneling nanotubes (TNTs)-F-actin-rich cytoplasmic connections between adjacent cells-and utilizes these structures for intercellular transmission. In this study, we used lentiviral transfection to express various structural and non-structural proteins of BVDV and identified NS5A as a critical viral protein that induces the formation of TNTs. RNA-seq analysis revealed that CKAP2, a host protein, plays a key role in TNT generation, with the PI3K/AKT/mTOR signaling pathway being essential for this process. Further investigation demonstrated that CKAP2 interacts with BVDV NS5A, triggering the activation of the PI3K/AKT/mTOR pathway, thereby promoting TNT formation and enhancing viral dissemination. Our data highlight a previously unknown mechanism of BVDV spreading and replication, which could have significant implications for within-host spread and immune evasion.

Indexed as

bovine viral diarrhea virusCKAP2NS5API3K/AKT/mTORtunneling nanotubes

Identifiers

PMID42357703
PMCPMC13307808

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.