ArticleViruses2026
Validation of an In-House High-Throughput Total RNA Sequencing Test for the Detection of Plant Viruses and Viroids.
Article in Viruses, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
1 citing paper in PubMed.
- A Validation Study of a High-Throughput Sequencing Workflow for the Detection of Grapevine Viruses and Viroids.Pathogens (Basel, Switzerland) · 2026Article
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Authors and funding
5 authors.
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No grant is acknowledged in the PubMed record.
Abstract
High-throughput sequencing is becoming the method of choice for plant diagnostics. It allows the detection of known and novel viruses and viroids, even in co-infection, without preliminary knowledge of the target. However, this method has its own limitations when compared to real-time PCR or ELISA. Laboratories that implement this type of technologies in-house must ensure that the performance criteria meet the requirements associated with their diagnostic activity. In this study, we present a workflow for in-house plant viruses and viroid detection, based on total RNA extraction, ribodepletion, Illumina sequencing and bioinformatics analyses. Performance criteria such as analytical sensitivity, analytical specificity, selectivity, repeatability, reproducibility and robustness were evaluated on the tomato brown rugose fruit virus (RNA genome), the tomato leaf curl New Delhi virus (DNA genome), and the pepper chat fruit viroid (RNA genome). The performance levels obtained meet the requirements for virus and viroid detection in symptomatic plant samples.
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