Mapping the TAR vRNA Interaction with HIV-1 Integrase.

Jian Sun, Rahul Yadav, Tolga Catmakas, Luke Fisher, Nicholas C Fitzkee, Jacques J Kessl

Abstract read

In one paragraph

Article in Viruses, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

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2 · The registry

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3 · Its place in the literature

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0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

Update of
Mapping the vRNA Interaction with HIV-1 Integrase.2026

5 · Who and what money

Authors and funding

6 authors.

Jian SunDepartment of Chemistry and Biochemistry, University of Southern Mississippi, Hattiesburg, MS 39406, USA.

Rahul YadavDepartment of Chemistry, Mississippi State University, Mississippi State, MS 39762, USA.

Tolga CatmakasDepartment of Chemistry and Biochemistry, University of Southern Mississippi, Hattiesburg, MS 39406, USA.

Luke FisherDepartment of Chemistry and Biochemistry, University of Southern Mississippi, Hattiesburg, MS 39406, USA.

Nicholas C FitzkeeDepartment of Chemistry, Mississippi State University, Mississippi State, MS 39762, USA.ORCID 0000-0002-8993-2140

Jacques J KesslDepartment of Chemistry and Biochemistry, University of Southern Mississippi, Hattiesburg, MS 39406, USA.ORCID 0000-0001-8103-6678

Funding

New Inhibitors Targeting HIV-1 Integrase During Viral MaturationR01AI140985 · NIAID · UNIVERSITY OF SOUTHERN MISSISSIPPI · PI Jacques J. Kessl · 2019 to 2026

$2.2M

NIAID NIH HHS R01 AI140985NIH HHS 2R01AI140985-05

6 · The paper itself

Abstract

A series of critical interactions within the viral core between viral RNA (vRNA) and HIV-1 Integrase (IN) has previously been reported. In these studies, contact points between vRNA and IN were identified using RNA-seq and MS-based protein foot-printing approaches. Several IN amino acids located in its C-terminal domain (CTD) were found to be essential for vRNA binding, and their alanine substitution severely impacted the correct morphogenesis of the mature viral core. Here, we have used the TAR element to extend these studies by performing a comprehensive mapping of the interaction by deploying RNA crosslinking and NMR methodologies. Together, these approaches were able to identify additional contact points between the TAR vRNA and IN. Our results reveal several new basic amino acids located in the IN CTD critical for the vRNA-IN interaction, viral replication and correct morphology of the mature viral core.

Indexed as

HIV-1HIV IntegraseHIV Long Terminal RepeatRNA, ViralHumansProtein BindingVirus ReplicationHIV Integrasep31 integrase protein, Human immunodeficiency virus 1RNA, ViralHIVintegrasevirus maturationvRNA

Identifiers

PMID42357667

DOI10.3390/v18060657

PMCPMC13307726

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.