ArticleViruses2026
Diagnostic Performance of PCR-Based Detection of Human Herpesvirus 8 DNA in Archived Formalin Fixed Paraffin Embedded Biopsy Specimens of Kaposi Sarcoma.
Article in Viruses, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
backgroundKaposi sarcoma (KS) is a vascular malignancy closely associated with human herpesvirus 8 (HHV-8) infection, and its diagnosis relies on combined clinical, histopathological and molecular assessment. This study aimed to detect HHV-8 DNA in archived formalin fixed paraffin embedded (FFPE) biopsy samples of KS by real time PCR and to evaluate the diagnostic performance of PCR compared with histopathology. Meth ods: In this retrospective cross-sectional study, 98 FFPE biopsy specimens with histopathologically confirmed KS and 30 FFPE biopsy specimens with non-KS vascular lesions were included as the patient and control groups, respectively. HHV-8 DNA was analyzed in all samples using real-time PCR. Diagnostic performance parameters, including sensitivity, specificity, predictive values, and accuracy, were calculated, and agreement with histopathological diagnosis was assessed using Cohen's kappa coefficient.
resultsHHV-8 PCR was positive in 89.8% (88/98) of KS cases and negative in all controls (30/30; 100%). Sensitivity, specificity, positive predictive value, negative predictive value and overall accuracy were 89.8%, 100%, 100%, 75.0% and 92.2%, respectively. Overall agreement between PCR and histopathology was 92.2% with a Cohen's kappa of 0.34 (
conclusionsHHV-8 PCR in archived FFPE KS biopsies shows high specificity and good sensitivity and acts as a complementary diagnostic tool with fair agreement with histopathology. It is particularly valuable in diagnostic gray areas but should always be interpreted together with clinical, histopathological and immunohistochemical findings.
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