Evidence map›Paper›PMID 42357611›Full record

ArticleViruses2026

Sodium Pyruvate Supplementation Enhances Infectious Yield and Supports Host-Cell Stability of Rabies Virus CVS-11 in a High-Density Macrocarrier-Based Tide-Motion Culture System.

Tolganay Imanbekova, Nurlan Akhmetsadykov, Bakdaulet Shanbayev, Zhanat Batanova, Ernur Nurolda, Yerkin Krykbayev, Anara Nurmukhambetova, Hsian-Yu Wang, Yu-Jing Zeng

Abstract read
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In one paragraph

Article in Viruses, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
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0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Tolganay ImanbekovaKazakh National Agrarian Research University, 8 Abai Avenue, Almaty 050010, Kazakhstan.
Nurlan AkhmetsadykovResearch and Production Enterprise Antigen LLP, Azerbayev Str. 4, Almaty Region, Settlement Abay, Karasai District, Almaty 040905, Kazakhstan.
Bakdaulet ShanbayevResearch and Production Enterprise Antigen LLP, Azerbayev Str. 4, Almaty Region, Settlement Abay, Karasai District, Almaty 040905, Kazakhstan.
Zhanat BatanovaKazakh National Agrarian Research University, 8 Abai Avenue, Almaty 050010, Kazakhstan.
Ernur NuroldaResearch and Production Enterprise Antigen LLP, Azerbayev Str. 4, Almaty Region, Settlement Abay, Karasai District, Almaty 040905, Kazakhstan.
Yerkin KrykbayevResearch and Production Enterprise Antigen LLP, Azerbayev Str. 4, Almaty Region, Settlement Abay, Karasai District, Almaty 040905, Kazakhstan.
Anara NurmukhambetovaAsfendiyarov Kazakh National Medical University, Tole Bi Street 94, Almaty 050012, Kazakhstan.
Hsian-Yu WangInstitute of Animal Vaccine Technology, National Pingtung University of Scientific and Technology, Taiwan R.O.C. 1, Shuefu Road, Neipu, Pingtung 912301, Taiwan.ORCID 0000-0002-7060-3005
Yu-Jing ZengInstitute of Animal Vaccine Technology, National Pingtung University of Scientific and Technology, Taiwan R.O.C. 1, Shuefu Road, Neipu, Pingtung 912301, Taiwan.ORCID 0009-0001-3168-341X

Funding

Science Committee, Ministry of Science and Higher Education, Republic of Kazakhstan AP19680178
6 · The paper itself

Abstract

Efficient in vitro production of rabies virus is essential for vaccine development and quality control applications. High-density cultivation systems offer practical advantages for rabies virus production but also create culture conditions in which nutrient depletion, waste accumulation, and progressive deterioration of host-cell condition may limit infectious virus output. In this study, we evaluated the effects of sodium pyruvate supplementation on rabies virus CVS-11 production in Vero and BSR cells cultivated in a high-density macrocarrier-based tide-motion culture system under serum-containing and serum-free conditions, with complementary comparative observations in conventional monolayer cultures of BHK cells. Cultures were infected at a multiplicity of infection of 0.01, and infectious virus production was assessed over time, together with cell density, glucose consumption, and pH dynamics. Sodium pyruvate supplementation was associated with significantly higher infectious virus titers, delayed culture deterioration, prolonged maintenance of viable cell populations, and higher peak infectious titers in both Vero and BSR cultures. The highest infectious titers were observed under serum-free pyruvate-supplemented conditions, reaching 7.5 log

Indexed as

Cell Culture TechniquesPyruvic AcidRabies virusVirus CultivationAnimalsCell LineChlorocebus aethiopsCricetinaeCulture MediaVero CellsVirus ReplicationCulture MediaPyruvic AcidBSR cellsCVS-11high-density culturemetabolic modulationrabies virusserum-free culturesodium pyruvateVero cells

Identifiers

PMID42357611
PMCPMC13307594

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.