P450 Fusion Protein Expressed in

Kinga Dulak, Agata Matera, Sandra Sordon, Maciej Wolak, Kinga Hyla, Ewa Huszcza, Jarosław Popłoński

Abstract read

In one paragraph

Article in Molecules (Basel, Switzerland), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Kinga DulakDepartment of Food Chemistry and Biocatalysis, Wroclaw University of Environmental and Life Sciences, 50-375 Wroclaw, Poland.ORCID 0000-0002-3642-0520

Agata MateraDepartment of Food Chemistry and Biocatalysis, Wroclaw University of Environmental and Life Sciences, 50-375 Wroclaw, Poland.

Sandra SordonDepartment of Food Chemistry and Biocatalysis, Wroclaw University of Environmental and Life Sciences, 50-375 Wroclaw, Poland.ORCID 0000-0001-7367-619X

Maciej WolakDepartment of Food Chemistry and Biocatalysis, Wroclaw University of Environmental and Life Sciences, 50-375 Wroclaw, Poland.ORCID 0009-0008-9091-9729

Kinga HylaDepartment of Biotechnology and Food Microbiology, Wroclaw University of Environmental and Life Sciences, 50-375 Wroclaw, Poland.ORCID 0000-0002-1437-114X

Ewa HuszczaDepartment of Food Chemistry and Biocatalysis, Wroclaw University of Environmental and Life Sciences, 50-375 Wroclaw, Poland.ORCID 0000-0002-5950-3695

Jarosław PopłońskiDepartment of Food Chemistry and Biocatalysis, Wroclaw University of Environmental and Life Sciences, 50-375 Wroclaw, Poland.ORCID 0000-0002-7254-5937

Funding

European Union 814650Wrocław University of Environmental and Life Sciences N070/0006/23

6 · The paper itself

Abstract

Plant cytochrome P450 monooxygenases (CYPs) are valuable biocatalysts for the regioselective hydroxylation of aromatic compounds. However, their expression in bacterial hosts is hampered by poor solubility, membrane anchoring and the requirement for redox partners. In this work, we report the design and characterization of modular expression systems that enable the functional production of SbCYP82D1.1 from

Indexed as

Cytochrome P-450 Enzyme SystemEscherichia coliFlavonoidsRecombinant Fusion ProteinsArabidopsisFlavanonesHydroxylationPlant ProteinsScutellaria baicalensisbaicaleinchrysinCytochrome P-450 Enzyme SystemFlavanonesFlavonoidsPlant ProteinsRecombinant Fusion Proteinsbaicaleinflavonoid hydroxylationlinkers designN-terminal modificationP450protein engineering

Identifiers

PMID42357584

DOI10.3390/molecules31122189

PMCPMC13304987

What OpenQuestion holds

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LicenceCC BY

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.