RNA Interference Silences Genes at Post-Transcriptional Level Without Impacting Nascent RNA in Soybean Hairy Roots.

Li Jiang, Jinfeng Pang, Qianyue Bai, Jian Wei, Zhicheng Dong

Abstract read

In one paragraph

Article in Plants (Basel, Switzerland), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Li JiangGuangzhou Key Laboratory of Crop Gene Editing, Guangdong Key Laboratory of Plant Adaptation and Molecular Design, Innovative Center of Molecular Genetics and Evolution, School of Life Sciences, Guangzhou University, Guangzhou 510006, China.

Jinfeng PangGuangzhou Key Laboratory of Crop Gene Editing, Guangdong Key Laboratory of Plant Adaptation and Molecular Design, Innovative Center of Molecular Genetics and Evolution, School of Life Sciences, Guangzhou University, Guangzhou 510006, China.

Qianyue BaiSchool of Agriculture, Jilin Agricultural University, Changchun 130118, China.

Jian WeiSchool of Agriculture, Jilin Agricultural University, Changchun 130118, China.ORCID 0009-0008-1176-7107

Zhicheng DongGuangzhou Key Laboratory of Crop Gene Editing, Guangdong Key Laboratory of Plant Adaptation and Molecular Design, Innovative Center of Molecular Genetics and Evolution, School of Life Sciences, Guangzhou University, Guangzhou 510006, China.ORCID 0000-0003-2909-5720

Funding

Guangdong Basic and Applied Basic Research Foundation 2023A1515110254National Natural Science Foundation of China 32090061National Natural Science Foundation of China 32401755the National Key Research and Development Program, China 2023YFD1200601

6 · The paper itself

Abstract

RNA interference (RNAi) is an effective mechanism for reducing gene expression, therefore enabling the exploration of gene function. Its application in hairy root transient transformation facilitates rapid preliminary evaluation of gene function, specifically tackling the difficulties linked to steady transformation in soybean. The mechanisms of RNAi have been thoroughly investigated in model plants. Nonetheless, it remains ambiguous whether silencing proceeds at the transcriptional or post-transcriptional level in hairy roots. We analyzed RNA levels and phenotype by RNAi targeting four genes (

Indexed as

hairy root systemnascent RNARNA interferencesoybean

Identifiers

PMID42357129

DOI10.3390/plants15121810

PMCPMC13307295

What OpenQuestion holds

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LicenceCC BY

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.