Evidence map›Paper›PMID 42353528›Full record

ArticleAnimals : an open access journal from MDPI2026

Double Electroporation Combined with Zona Pellucida Removal Improves Biallelic Genome Editing Efficiency in Porcine Embryos.

Nanaka Torigoe, Takeshige Otoi, Manita Wittayarat, Oky Setyo Widodo, Theerawat Tharasanit, Kaywalee Chatdarong, Megumi Nagahara, Maki Hirata, Fuminori Tanihara, Zhao Namula

Abstract read
In one paragraph

Article in Animals : an open access journal from MDPI, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Nanaka TorigoeCollege of Coastal Agricultural Sciences, Guangdong Ocean University, Zhanjiang 524088, China.
Takeshige OtoiCollege of Coastal Agricultural Sciences, Guangdong Ocean University, Zhanjiang 524088, China.ORCID 0000-0002-0382-959X
Manita WittayaratFaculty of Veterinary Science, Prince of Songkla University, Songkhla 90110, Thailand.ORCID 0000-0003-1646-0581
Oky Setyo WidodoFaculty of Veterinary Medicine, Universitas Airlangga, Surabaya 60115, Indonesia.ORCID 0000-0002-0073-1394
Theerawat TharasanitFaculty of Veterinary Science, Chulalongkorn University, Bangkok 10330, Thailand.ORCID 0000-0002-0225-5854
Kaywalee ChatdarongFaculty of Veterinary Science, Chulalongkorn University, Bangkok 10330, Thailand.ORCID 0000-0002-9470-8426
Megumi NagaharaCollege of Coastal Agricultural Sciences, Guangdong Ocean University, Zhanjiang 524088, China.ORCID 0009-0009-7045-3922
Maki HirataCollege of Coastal Agricultural Sciences, Guangdong Ocean University, Zhanjiang 524088, China.ORCID 0000-0003-4673-1581
Fuminori TaniharaCollege of Coastal Agricultural Sciences, Guangdong Ocean University, Zhanjiang 524088, China.
Zhao NamulaCollege of Coastal Agricultural Sciences, Guangdong Ocean University, Zhanjiang 524088, China.

Funding

Japan Society for the Promotion of Science JP21KK0124, JP22K19896, JP23K23764, and JP23K23805Key Laboratory of Guangdong Province 2022B0202090003
6 · The paper itself

Abstract

The CRISPR/Cas9 system has been widely used for gene editing in various species; however, mosaicism remains a significant challenge. This study aimed to improve gene editing efficiency and reduce mosaicism in porcine embryos by exploring double electroporation pre- and post-in vitro fertilization combined with zona pellucida (ZP) removal. We evaluated the effects of these treatments on the development and mutation rates of oocytes/zygotes edited with guide RNAs (gRNAs) targeting

Indexed as

double electroporationgene editingmosaic mutationpigzona free

Identifiers

PMID42353528
PMCPMC13295827

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.