Engineering a Fungal Non-Reducing Polyketide Synthase with an Apparently Inactive Product-Template Domain Reveals Insights into the Catalytic Reprogramming.

Ruya Yin, Yifei Qin, Xingrui Liang, Ziqi Zhai, Mengwei Zhang, Dan Xu, Ligang Zhou, Daowan Lai

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Article in International journal of molecular sciences, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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8 authors.

Ruya YinState Key Laboratory of Agricultural and Forestry Biosecurity, MARA Key Lab of Pest Monitoring and Green Management, College of Plant Protection, China Agricultural University, Beijing 100193, China.

Yifei QinState Key Laboratory of Agricultural and Forestry Biosecurity, MARA Key Lab of Pest Monitoring and Green Management, College of Plant Protection, China Agricultural University, Beijing 100193, China.

Xingrui LiangState Key Laboratory of Agricultural and Forestry Biosecurity, MARA Key Lab of Pest Monitoring and Green Management, College of Plant Protection, China Agricultural University, Beijing 100193, China.

Ziqi ZhaiState Key Laboratory of Agricultural and Forestry Biosecurity, MARA Key Lab of Pest Monitoring and Green Management, College of Plant Protection, China Agricultural University, Beijing 100193, China.

Mengwei ZhangState Key Laboratory of Agricultural and Forestry Biosecurity, MARA Key Lab of Pest Monitoring and Green Management, College of Plant Protection, China Agricultural University, Beijing 100193, China.

Dan XuState Key Laboratory of Agricultural and Forestry Biosecurity, MARA Key Lab of Pest Monitoring and Green Management, College of Plant Protection, China Agricultural University, Beijing 100193, China.

Ligang ZhouState Key Laboratory of Agricultural and Forestry Biosecurity, MARA Key Lab of Pest Monitoring and Green Management, College of Plant Protection, China Agricultural University, Beijing 100193, China.ORCID 0000-0003-4681-191X

Daowan LaiState Key Laboratory of Agricultural and Forestry Biosecurity, MARA Key Lab of Pest Monitoring and Green Management, College of Plant Protection, China Agricultural University, Beijing 100193, China.ORCID 0000-0001-7984-5855

Funding

National Natural Science Foundation of China 31872623

6 · The paper itself

Abstract

Fungal iterative non-reducing polyketide synthases (NR-PKS) contain a unique product template (PT) domain for aromatic cyclization. Among them, some NR-PKSs, such as the sorbicillin NR-PKS (SorB), have an apparently inactive PT. It is unknown what role such PT plays in NR-PKS programming. In this study, the PT domain of SorB was first dissected and engineered. Removal of the PT domain from SorB did not change the product profile, but the yield decreased. Meanwhile, a significantly lower transcriptional level of the ketoacyl synthase (KS) domain was observed in the knockout mutant (

Indexed as

Fungal ProteinsPolyketide SynthasesProtein EngineeringAmino Acid SequenceCatalytic DomainMutationPhylogenyProtein DomainsFungal ProteinsPolyketide Synthasesbiosynthesiscatalytic reprogrammingC-methyltransferasedomain engineeringpolyketidepolyketide synthaseproduct templatesorbicillinsynthetic biology

Identifiers

PMID42353248

DOI10.3390/ijms27125534

PMCPMC13300579

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