The Anticonvulsant Effect of Long-Term Valproate Might Be Attributable to Altered Expression of Selected Genes in Mice.

Monika Banach, Przemysław Kołodziej, Jacek Bogucki, Kinga Borowicz, Anna Bogucka-Kocka

Abstract read

In one paragraph

Article in International journal of molecular sciences, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Monika BanachIndependent Experimental Neuropathophysiology Unit, Department of Toxicology, Faculty of Pharmacy, Medical University of Lublin, Jaczewskiego 8b Street, 20-090 Lublin, Poland.ORCID 0000-0003-4614-1071

Przemysław KołodziejDepartment of Biology and Genetics, Faculty of Pharmacy, Medical University of Lublin, 4A Chodzki Street, 20-093 Lublin, Poland.ORCID 0000-0002-3710-0860

Jacek BoguckiDepartment of Research Methodology in Medicine, Institute of Medical Biology, Faculty of Medicine, The John Paul II Catholic University of Lublin, Konstantynów 1H St., 20-708 Lublin, Poland.ORCID 0000-0002-8683-545X

Kinga BorowiczIndependent Experimental Neuropathophysiology Unit, Department of Toxicology, Faculty of Pharmacy, Medical University of Lublin, Jaczewskiego 8b Street, 20-090 Lublin, Poland.

Anna Bogucka-KockaDepartment of Biology and Genetics, Faculty of Pharmacy, Medical University of Lublin, 4A Chodzki Street, 20-093 Lublin, Poland.

Funding

Medical University of Lublin DS43Medical University of Lublin DS57

6 · The paper itself

Abstract

In this study, we assessed the impact of repeated valproate administration on its anticonvulsant effects and side effects in mice. We measured the plasma and brain concentrations of valproate and examined changes in the expression of selected genes in the mouse hippocampus after both acute and chronic treatments. Electroconvulsions were induced using an alternating current (50 Hz, 25 mA, 0.2 s) through ear clip electrodes. Motor impairment and long-term memory deficits were assessed with the chimney test and passive avoidance task. Valproate concentrations in the brain and plasma were measured by a fluorescence polarization immunoassay. mRNA was isolated using a modified Chomczyński and Sacchi method, and RQ-PCR was performed with an Applied Biosystems 7900 using SDS and RQ Study software. The 50% effective dose (ED

Indexed as

AnticonvulsantsGene Expression RegulationSeizuresValproic AcidAnimalsBrainElectroshockGlutamate DecarboxylaseHippocampusMaleMiceNAV1.1 Voltage-Gated Sodium ChannelAnticonvulsantsGlutamate Decarboxylaseglutamate decarboxylase 1NAV1.1 Voltage-Gated Sodium ChannelValproic Acidantiepileptic drugschronic treatmentepilepsygene expressionmaximal electroshock-induced seizuresvalproate

Identifiers

PMID42353001

DOI10.3390/ijms27125281

PMCPMC13300035

What OpenQuestion holds

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.