ArticleInternational journal of molecular sciences2026
Expanding Steroid Glycodiversity: Tandem Steroid Glucosylation and Acetylation via Enzymatic Cascade.
Article in International journal of molecular sciences, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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6 authors.
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Abstract
Steroid glycosides constitute an important class of bioactive molecules, yet their selective synthesis remains challenging. Here, we established a screening platform for nucleotide sugar-dependent glycosyltransferases (GTs) coupled with sucrose synthase (SuSy) for in situ UDP-glucose regeneration, enabling cost-efficient steroid glucosylation. A library of GTs comprising literature-derived enzymes and newly mined archaeal and fungal candidates was constructed using sequence filtering, AlphaFold3 modeling, and docking-guided prioritization. The resulting panel was screened against 31 structurally diverse steroids (androgens, estrogens, pregnanes, and corticosteroids) using crude
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