Evidence map›Paper›PMID 42351229›Full record

ArticleBMC research notes2026

The M91 metallopeptidase domain of the T6SS effector protein SED_RS06335 encoded in Salmonella Dublin SPI-19 exhibits antibacterial activity.

Carla Vargas-Del Río, Ayleen Parra-Calisto, Carlos J Blondel, Felipe Reyes-Méndez, Fernando A Amaya, Carlos A Santiviago, Andrea Avilés, Victoria Soriano-Mora, Daniel Chacín, David Pezoa

Abstract read
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In one paragraph

Article in BMC research notes, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0cells of the map it votes in
0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Carla Vargas-Del Río *Núcleo de Microbiología Traslacional para la Vigilancia e Innovación en Sistemas Sanitarios, Ambientales y Productivos MICRA, Facultad de Medicina Veterinaria y Agronomía, Universidad de Las Américas, Campus Providencia, Manuel Montt 948, Santiago, Chile.
Ayleen Parra-Calisto *Núcleo de Microbiología Traslacional para la Vigilancia e Innovación en Sistemas Sanitarios, Ambientales y Productivos MICRA, Facultad de Medicina Veterinaria y Agronomía, Universidad de Las Américas, Campus Providencia, Manuel Montt 948, Santiago, Chile.
Carlos J BlondelInstitute of Biomedical Sciences, Faculty of Medicine, Universidad Andres Bello, Santiago, Chile.ORCID http://orcid.org/0000-0002-7099-6347
Felipe Reyes-MéndezNúcleo de Microbiología Traslacional para la Vigilancia e Innovación en Sistemas Sanitarios, Ambientales y Productivos MICRA, Facultad de Medicina Veterinaria y Agronomía, Universidad de Las Américas, Campus Providencia, Manuel Montt 948, Santiago, Chile.
Fernando A AmayaLaboratorio de Microbiología, Departamento de Bioquímica y Biología Molecular, Facultad de Ciencias Químicas y Farmacéuticas, Universidad de Chile, Santiago, Chile.ORCID http://orcid.org/0000-0001-6424-108X
Carlos A SantiviagoLaboratorio de Microbiología, Departamento de Bioquímica y Biología Molecular, Facultad de Ciencias Químicas y Farmacéuticas, Universidad de Chile, Santiago, Chile.ORCID http://orcid.org/0000-0002-7043-7908
Andrea AvilésLaboratorio de Microbiología, Departamento de Bioquímica y Biología Molecular, Facultad de Ciencias Químicas y Farmacéuticas, Universidad de Chile, Santiago, Chile.
Victoria Soriano-MoraNúcleo de Microbiología Traslacional para la Vigilancia e Innovación en Sistemas Sanitarios, Ambientales y Productivos MICRA, Facultad de Medicina Veterinaria y Agronomía, Universidad de Las Américas, Campus Providencia, Manuel Montt 948, Santiago, Chile.
Daniel ChacínNúcleo de Microbiología Traslacional para la Vigilancia e Innovación en Sistemas Sanitarios, Ambientales y Productivos MICRA, Facultad de Medicina Veterinaria y Agronomía, Universidad de Las Américas, Campus Providencia, Manuel Montt 948, Santiago, Chile.
David PezoaNúcleo de Microbiología Traslacional para la Vigilancia e Innovación en Sistemas Sanitarios, Ambientales y Productivos MICRA, Facultad de Medicina Veterinaria y Agronomía, Universidad de Las Américas, Campus Providencia, Manuel Montt 948, Santiago, Chile. dpezoa@udla.cl.ORCID http://orcid.org/0009-0005-7872-2374

Funding

ECOS-ANID ECOS200037FONDECYT grant 11240160FONDECYT grant 1212075FONDECYT grant 1241637HHMI-Gulbenkian International Research Scholar Grant 55008749
6 · The paper itself

Abstract

objectiveThe Type VI Secretion System (T6SS) is a contractile apparatus made of several proteins playing a significant role in the fitness and virulence of many Gram-negative bacteria. The SPI-19 T6SS gene cluster is a virulence factor of Salmonella Dublin contributing to host colonization and antibacterial activity. Previously, we demonstrated that SED_RS06335 (an Rhs protein with a C-terminal M91 domain) is responsible for antibacterial activity of the T6SS

resultsHere, we determine through interbacterial competition and heterologous expression assays that M91 domain of SED_RS06335 displays antibacterial activity. Furthermore, a three-dimensional structural model of M91 domain revealed a high similarity with the metallopeptidase neurotoxin type A (BotA) from Clostridium botulinum. Interestingly, our results suggest that the M91 domain is responsible for the antibacterial activity of the SED_RS06335 effector in Salmonella Dublin.

Indexed as

Anti-Bacterial AgentsBacterial ProteinsMetalloproteasesSalmonellaType VI Secretion SystemsModels, MolecularProtein DomainsAnti-Bacterial AgentsBacterial ProteinsMetalloproteasesType VI Secretion SystemsAntibacterial activityM91 metallopeptidase domainSalmonella DublinSED_RS06335T6SS

Identifiers

PMID42351229
PMCPMC13556075

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.