Disrupting intestinal cell pyroptosis-NETs crosstalk reverse mucosal damage and restore Treg/Th17 equilibrium in ulcerative colitis via a calcium pectate microsphere composite hydrogel.

Yun Huang, Shuangyan He, Jia Liu, Yalan Chen, Wen Chen, Mohan Ding, Zhi Zheng, Hua Wei, Cui-Yun Yu

Abstract read

In one paragraph

Article in Journal of nanobiotechnology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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3 · Its place in the literature

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0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

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5 · Who and what money

Authors and funding

9 authors.

Yun Huang *Hunan Province Cooperative Innovation Center for Molecular Target New Drug Study, School of Pharmaceutical Science, Hengyang Medical School, University of South China, Hengyang, 421001, China.

Shuangyan He *Hunan Province Cooperative Innovation Center for Molecular Target New Drug Study, School of Pharmaceutical Science, Hengyang Medical School, University of South China, Hengyang, 421001, China.

Jia LiuHunan Province Cooperative Innovation Center for Molecular Target New Drug Study, School of Pharmaceutical Science, Hengyang Medical School, University of South China, Hengyang, 421001, China.

Yalan ChenHunan Province Cooperative Innovation Center for Molecular Target New Drug Study, School of Pharmaceutical Science, Hengyang Medical School, University of South China, Hengyang, 421001, China.

Wen ChenHunan Province Cooperative Innovation Center for Molecular Target New Drug Study, School of Pharmaceutical Science, Hengyang Medical School, University of South China, Hengyang, 421001, China.

Mohan DingHunan Province Cooperative Innovation Center for Molecular Target New Drug Study, School of Pharmaceutical Science, Hengyang Medical School, University of South China, Hengyang, 421001, China.

Zhi ZhengHunan Province Cooperative Innovation Center for Molecular Target New Drug Study, School of Pharmaceutical Science, Hengyang Medical School, University of South China, Hengyang, 421001, China.

Hua WeiHunan Province Cooperative Innovation Center for Molecular Target New Drug Study, School of Pharmaceutical Science, Hengyang Medical School, University of South China, Hengyang, 421001, China. weih@usc.edu.cn.

Cui-Yun YuAffiliated Hospital of Hunan Academy of Chinese Medicine, Hunan Academy of Chinese Medicine, Changsha, 410013, China. yucuiyunusc@hotmail.com.

Funding

National Natural Science Foundation of China 82373826

6 · The paper itself

Abstract

The crosstalk between dysregulated intestinal epithelial cells (IECs) death and hyperactivated immune cells is crucial to maintain the intestinal barrier integrity in ulcerative colitis (UC), but remains seldom explored for the anti-inflammatory therapies reported so far. For this purpose, this study first identifies the crucial role of the crosstalk between Gasdermin D (GSDMD)-dependent intestinal cell pyroptosis and neutrophil extracellular traps (NETs) in exacerbating intestinal barrier damage. An orally administrable inulin gel composite (BCPD@gel) was further developed, encapsulating calcium pectate-based microspheres that are loaded simultaneously with berberine (BBR) and Deoxyribonuclease I (DNase I) via hydrophobic and electrostatic interactions, respectively. BCPD@gel breaks the crosstalk between GSDMD-dependent intestinal cell pyroptosis and NETs to modulate gut microbiota composition and restore Th17/Treg equilibrium, collectively leading to compromised inflammation cascade for dextran sulfate sodium (DSS)-induced colitis treatment. BCPD@gel administration greatly restored epithelium barriers by alleviating the vicious cycle between GSDMD-dependent IEC pyroptosis and NETs in a DSS-induced colitis model, which was evidenced by a mouse colon length of 6.76 ± 0.4 cm. This improved value is close to that of the normal group and 1.4-fold longer than that of the DSS group. Overall, this study highlights the leverage of IEC-neutrophil interactions as a potent strategy to circumvent ongoing inflammation and maintain the intestinal barrier integrity for effective DSS-induced colitis treatment.

Indexed as

Colitis, UlcerativeExtracellular TrapsHydrogelsIntestinal MucosaPyroptosisAnimalsDextran SulfateGasderminsHumansIntestinal Barrier FunctionMaleMiceMice, Inbred C57BLMicrospheresPhosphate-Binding ProteinsTh17 CellsDextran SulfateGasderminsGsdmd protein, mouseHydrogelsPhosphate-Binding ProteinsIntestinal barrier integrityNeutrophil extracellular trapsPyroptosisUlcerative colitisVicious cycle

Identifiers

PMID42351171

DOI10.1186/s12951-026-04743-1

PMCPMC13563845

What OpenQuestion holds

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.