Evidence map›Paper›PMID 42350861›Full record

ArticleInternational microbiology : the official journal of the Spanish Society for Microbiology2026

Revealing 3-hydroxybenzoate-4-hydroxylase from Pseudarthrobacter phenanthrenivorans Sphe3 via In-Cell NMR analysis and molecular cloning.

Stamatia Asimakoula, Alexandra Primikyri, Epameinondas Tsagogiannis, Anna-Irini Koukkou

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Article in International microbiology : the official journal of the Spanish Society for Microbiology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Stamatia AsimakoulaLaboratory of Biochemistry, Sector of Organic Chemistry and Biochemistry, Department of Chemistry, University of Ioannina, Ioannina, 45110, Greece.
Alexandra PrimikyriLaboratory of Organic Chemistry, Sector of Organic Chemistry and Biochemistry, Department of Chemistry, University of Ioannina, Ioannina, 45110, Greece. a.primikyri@uoi.gr.
Epameinondas TsagogiannisLaboratory of Biochemistry, Sector of Organic Chemistry and Biochemistry, Department of Chemistry, University of Ioannina, Ioannina, 45110, Greece.
Anna-Irini KoukkouLaboratory of Biochemistry, Sector of Organic Chemistry and Biochemistry, Department of Chemistry, University of Ioannina, Ioannina, 45110, Greece. akukku@uoi.gr.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

This study aims at the functional and kinetic characterization of 3-Hydroxybenzoate-4-Hydroxylase (3HB4H) from Pseudarthrobacter phenanthrenivorans Sphe3. Bioinformatic sequence and structural analyses suggested that Asphe3_36590 gene encodes a putative 3HB4H, a finding supported by transcriptional data of a ~ 2000-fold induction when Sphe3 cells were grown on 3-Hydroxybenzoate (3-HBA) compared to glucose. The recombinant 3HB4H is highly specific for 3-HBA and follows Michaelis-Menten kinetics, with Km and Vmax values of 72.6 µM and 42.5 µM × min

Indexed as

ActinobacteriaBacterial ProteinsProtocatechuate-3,4-DioxygenaseCloning, MolecularEscherichia coliGentisatesHydroxybenzoatesKineticsMagnetic Resonance SpectroscopyParabensSubstrate Specificity3-hydroxybenzoic acid4-hydroxybenzoic acidBacterial ProteinsGentisatesHydroxybenzoatesParabensProtocatechuate-3,4-Dioxygenaseprotocatechuic acid3-hydroxybenzoate 4-hydroxylaseActinobacteriaFlavoprotein monooxygenasesIn-cell NMRProtocatechuic acidPseudarthrobacter phenanthrenivorans Sphe3

Identifiers

PMID42350861
PMCPMC13653839

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.