Evidence map›Paper›PMID 42349406›Full record

ArticleNeuron2026

A two-timepoint framework for sensitive and specific single-cell activity screening.

Alejandro Ramirez, Evan J Kyzar, Lydia Rogerson, Chloé Berland, Erica Rodriguez, Juan Guerrero, Ruby Setara, Maya Eisengart, Sophia Virkar, Luke A Hammond and 2 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Neuron, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Lasting aversive consequences of a single dose of 4-hydroxytamoxifen.bioRxiv : the preprint server for biology · 2026
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Alejandro RamirezDepartment of Neuroscience, Columbia University, New York, NY, USA; Mortimer B. Zuckerman Mind Brain Behavior Institute, Columbia University, New York, NY, USA; Department of Psychiatry, Columbia University, New York, NY, USA; New York State Psychiatric Institute, New York, NY, USA.
Evan J KyzarDepartment of Neuroscience, Columbia University, New York, NY, USA; Mortimer B. Zuckerman Mind Brain Behavior Institute, Columbia University, New York, NY, USA; Department of Psychiatry, Columbia University, New York, NY, USA; New York State Psychiatric Institute, New York, NY, USA.
Lydia RogersonDepartment of Neuroscience, Columbia University, New York, NY, USA; Mortimer B. Zuckerman Mind Brain Behavior Institute, Columbia University, New York, NY, USA.
Chloé BerlandDepartment of Medicine, Columbia University, New York, NY, USA; Naomi Berrie Diabetes Center, Columbia University, New York, NY, USA.
Erica RodriguezDepartment of Neuroscience, Columbia University, New York, NY, USA; Mortimer B. Zuckerman Mind Brain Behavior Institute, Columbia University, New York, NY, USA.
Juan GuerreroDepartment of Neuroscience, Columbia University, New York, NY, USA; Mortimer B. Zuckerman Mind Brain Behavior Institute, Columbia University, New York, NY, USA.
Ruby SetaraDepartment of Neuroscience, Columbia University, New York, NY, USA; Mortimer B. Zuckerman Mind Brain Behavior Institute, Columbia University, New York, NY, USA.
Maya EisengartDepartment of Neuroscience, Columbia University, New York, NY, USA; Mortimer B. Zuckerman Mind Brain Behavior Institute, Columbia University, New York, NY, USA.
Sophia VirkarDepartment of Neuroscience, Columbia University, New York, NY, USA; Mortimer B. Zuckerman Mind Brain Behavior Institute, Columbia University, New York, NY, USA.
Luke A HammondDepartment of Neuroscience, Columbia University, New York, NY, USA; Mortimer B. Zuckerman Mind Brain Behavior Institute, Columbia University, New York, NY, USA.
Anthony W FerranteDepartment of Medicine, Columbia University, New York, NY, USA; Naomi Berrie Diabetes Center, Columbia University, New York, NY, USA.
C Daniel SalzmanDepartment of Neuroscience, Columbia University, New York, NY, USA; Mortimer B. Zuckerman Mind Brain Behavior Institute, Columbia University, New York, NY, USA; Department of Psychiatry, Columbia University, New York, NY, USA; New York State Psychiatric Institute, New York, NY, USA; Kavli Institute for Brain Sciences, Columbia University, New York, NY, USA. Electronic address: cds2005@columbia.edu.

Funding

Research Training in Mood and Anxiety Disorders: From Animal Models to PatientsT32MH015144 · NIMH · COLUMBIA UNIV NEW YORK MORNINGSIDE · PI Eduardo David Leonardo · 1985 to 2026
$11.1M
Neural Mechanisms that Underlie Eating in the Absence of HungerK08DK132493 · NIDDK · COLUMBIA UNIVERSITY HEALTH SCIENCES · PI RAMIREZ, ALEJANDRO · 2023 to 2024
$332k
Dissecting the synaptic and circuit mechanisms underlying olfactory-driven social behaviorK99GM153720 · NIGMS · COLUMBIA UNIVERSITY HEALTH SCIENCES · PI RODRIGUEZ, ERICA J · 2023 to 2024
$250k
NIDDK NIH HHS K08 DK132493NIGMS NIH HHS K99 GM153720NIMH NIH HHS L70 MH134315NIMH NIH HHS T32 MH015144
6 · The paper itself

Abstract

Methods to identify brain areas where neurons are activated in response to stimuli or states typically rely on immediate early gene (IEG) expression. However, variability in IEG expression across subjects and brain areas often requires large sample sizes. Further, IEG expression alone cannot determine whether the same or different neurons are activated in response to two distinct stimuli or states. To overcome these challenges, we developed a brain screening method-two-timepoint statistical inference with subtraction (TTP-S)-to identify brain areas on the basis of activity at two timepoints, not one. Applying this approach across 500+ brain areas increases the sensitivity and specificity of screens for engaged brain areas, thereby reducing the required sample size. Coupled with graph theoretical analyses, we use this method to analyze hunger, satiety, food cue presentation, drug treatment, and alcohol consumption, identifying known relevant brain regions and implicating new areas.

Indexed as

alcoholc-Fosfeedinghungerimagingsatietysemaglutidewhole-brain screening

Identifiers

PMID42349406
PMCPMC13334391

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.