ArticleClinical and applied thrombosis/hemostasis : official journal of the International Academy of Clinical and Applied Thrombosis/Hemostasis
Interpretation of Prothrombin Time and Activated Partial Thromboplastin Time Mixing Studies Using Predefined Algorithms.
Article in Clinical and applied thrombosis/hemostasis : official journal of the International Academy of Clinical and Applied Thrombosis/Hemostasis. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
BackgroundMixing test plasma with prolonged Prothrombin Time (PT) and activated partial thromboplastin time (aPTT) with normal plasma in a 1:1 ratio, helps distinguish between clotting factor deficiency and inhibitors. In our clinical laboratory, the upper reference interval (URI) is routinely used to determine correction (or no correction) in mixing studies.Aims and objectivesWe aimed to use the International Council for Standardization in Haematology. (ICSH) 2024 recommendations for interpreting PT/aPTT mixing studies following validation of the defined cutoffs.Materials and MethodsFrom December 2022 to December 2024, plasma samples with prolonged PT/aPTT were tested by mixing them with normal pooled plasma at a 1:1 ratio. A cut-off of ≥70% correction or ≤15% Rosner Index was used to identify clotting factor deficiency per ICSH, and this was compared with URI.ResultsAfter validating the cutoffs, the analysis of mixing results and factor assays was performed on 243 patients with prolonged PT (n=54), aPTT (n=56), and PT/aPTT (n=133). All patients exhibited either single or multiple clotting factor deficiencies. Inhibitor detection varied depending on the interpretive criteria: the upper reference interval method identified 12/243 cases (4.9%), the 70% correction criterion identified 20/243 cases (8.2%) overall, and the Rosner Index identified 7/189 cases (3.7%) among samples with prolonged aPTT, highlighting method-dependent discordance. Agreement between the various methods was 90.0% or higher.ConclusionThe ICSH recommendations accurately identified clotting factor deficiencies after local validation of the cut-offs. Clinical correlation and appropriate laboratory testing are essential for detecting concurrent inhibitors in clotting factor deficiency.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.