Evidence map›Paper›PMID 42348609›Full record

ArticlePloS one2026

Genomic characterization of multidrug-resistant Escherichia coli isolated from gills of Labeo rohita: Insight into resistome, virulence and pathogenicity.

Amit Kumar, Rahul Singh, Asma Masood, Dhouha Choukaier, Abhinav Kumar, Ankit Dilipkumar Oza, Denekew Alemayehu Alamneh

Abstract read
In one paragraph

Article in PloS one, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Amit KumarDepartment of Zoology, School of Bioengineering and Biosciences, Lovely Professional University Phagwara, Phagwara, Punjab India.
Rahul SinghDepartment of Zoology, School of Bioengineering and Biosciences, Lovely Professional University Phagwara, Phagwara, Punjab India.
Asma MasoodDepartment of Zoology, School of Bioengineering and Biosciences, Lovely Professional University Phagwara, Phagwara, Punjab India.
Dhouha ChoukaierDepartment of Basic Health Sciences in the Foundation Year for Health Colleges Program, College of Languages, Princess Nourah bint Abdulrahman University, Riyadh, Saudi Arabia.
Abhinav KumarSustainable Development Research Center, Azerbaijan State Oil and Industry University, Baku, Azerbaijan.
Ankit Dilipkumar OzaDepartment of Mathematical Sciences, Saveetha School of Engineering, SIMATS, Chennai, Tamilnadu, India.
Denekew Alemayehu AlamnehDepartment of Mathematics, Hawassa University, Hawassa, Ethiopia.ORCID https://orcid.org/0009-0003-3121-5872

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Antibiotic resistance in aquaculture settings is an emerging issue that threatens animal and human health. In this work, a multidrug-resistant (MDR) Escherichia coli strain (RG3) was isolated from the gill of Labeo rohita obtained from a retail fish market in Punjab, India. Antimicrobial susceptibility testing revealed resistance to various antibiotic groups, with intermediate susceptibility to imipenem. Whole-genome sequencing produced a 3.70 Mb draft genome with 3,814 genes and 39 resistance determinants. Genome analysis identified a chromosomally encoded multidrug resistance phenotype, including β-lactam resistance and efflux-mediated mechanisms, together with virulence factors associated with iron acquisition, biofilm formation, and host colonization. No plasmids were predicted in the short-read assembly. Genome-based prediction suggested a high likelihood of human pathogenicity and assigned the isolate to sequence type ST8020. This study aimed to characterize an MDR E. coli from an aquaculture source using phenotypic and genomic approaches. The identification of a chromosomally encoded resistome together with virulence-associated traits highlights the potential of aquaculture-derived E. coli to act as reservoirs of antimicrobial resistance within a one health framework.

Indexed as

CyprinidaeDrug Resistance, Multiple, BacterialEscherichia coliEscherichia coli InfectionsGillsAnimalsAnti-Bacterial AgentsGenome, BacterialMicrobial Sensitivity TestsVirulenceVirulence FactorsWhole Genome SequencingAnti-Bacterial AgentsVirulence Factors

Identifiers

PMID42348609
PMCPMC13298948

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.