Long-Term Impact of Early-Life Stress on Hippocampal Apoptotic Gene Expression in BALB/c Mice.

Aida Nurul Barokah, İhsan Kıvanç Gürsoy, Merve Hilal Dönmez, Juliette Fitremann, Arslan Bayram, Keziban Korkmaz Bayram

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Article in Neurochemical research, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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4 · The record

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5 · Who and what money

Authors and funding

6 authors.

Aida Nurul BarokahDepartment of Translational Medicine, Institute of Health Sciences, Ankara Yıldırım Beyazit University, Ankara, Türkiye.ORCID http://orcid.org/0000-0003-3102-7858

İhsan Kıvanç GürsoyDepartment of Medical Genetics, Faculty of Medicine, Ankara Yıldırım Beyazit University, Ankara, Türkiye.ORCID http://orcid.org/0009-0006-5393-4132

Merve Hilal DönmezDepartment of Translational Medicine, Institute of Health Sciences, Ankara Yıldırım Beyazit University, Ankara, Türkiye.ORCID http://orcid.org/0000-0001-6286-7038

Juliette FitremannSOFTMAT, Université de Toulouse, CNRS UMR 5623, Toulouse, France.ORCID http://orcid.org/0000-0002-1080-3742

Arslan BayramGENTAN, Genetic Diseases Evaluation Center, İzmir, Türkiye.ORCID http://orcid.org/0000-0002-3682-2140

Keziban Korkmaz BayramDepartment of Medical Genetics, Faculty of Medicine, Ankara Yıldırım Beyazit University, Ankara, Türkiye. keziban.korkmazbayram@ibg.edu.tr.ORCID http://orcid.org/0000-0002-1228-1298

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Early-life stress (ELS), such as maternal separation, has been associated with neuronal apoptosis and impaired hippocampal function in rodent models. This study investigated the long-term effects of two ELS paradigms-unpredictable maternal separation (MS) and MS combined with unpredictable maternal stress (MSUS)-on the hippocampal mRNA expression of proapoptotic (Bax, Tp53 and Casp3) and prosurvival (Bcl2) genes in offspring, both in vivo and in a complementary in vitro neuronal culture model. The study included three groups (Control, MS and MSUS groups), with ELS applied from postnatal days 1 to 14 to BALB/c mice. For the in vitro experiments, total RNA from the MSUS hippocampus was nucleofected at different concentrations into healthy mouse hippocampal cells, followed by 3D neuronal cell culture using N-heptyl-D-galactonamide (GalC7) hydrogels. Quantitative real-time PCR was used to assess gene expression, which was analyzed via the comparative Ct method (2

Indexed as

ApoptosisHippocampusMaternal DeprivationStress, PsychologicalAnimalsbcl-2-Associated X ProteinCaspase 3Cells, CulturedFemaleMiceMice, Inbred BALB CNeuronsProto-Oncogene Proteins c-bcl-2RNA, MessengerTumor Suppressor Protein p53bcl-2-Associated X ProteinCaspase 3Proto-Oncogene Proteins c-bcl-2RNA, MessengerTumor Suppressor Protein p533D hippocampal cell cultureApoptosisCell survivalMaternal deprivationNucleofection

Identifiers

PMID42347997

DOI10.1007/s11064-026-04822-7

PMCPMC13303556

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.