Evidence map›Paper›PMID 42347246›Full record

ArticlePathogens (Basel, Switzerland)2026

Direct-from-Specimen Detection of Major Carbapenemases by Carbapenem-Resistant K.N.I.V.O. Detection K-Set: Comparative Analysis of Accuracy and Turnaround Time.

Basant Mostafa Gabr, Mona Abd El-Aziz Gadallah, Wafaa Abd Elaziz, Sama Metwally, Raghda Gabr Mashaal, Rasha A Abd Ellatif, Ahmed G Elkhouly, Hanan Salem, Amira E Oraiby, Bsant S Kasem and 3 more

Abstract readComparative Study
PubMed Full text at PMC Publisher
In one paragraph

Article in Pathogens (Basel, Switzerland), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Basant Mostafa GabrDepartment of Medical Microbiology and Immunology, Faculty of Medicine, Tanta University, Tanta 31527, Egypt.ORCID 0000-0002-3509-0347
Mona Abd El-Aziz GadallahDepartment of Medical Microbiology and Immunology, Faculty of Medicine, Tanta University, Tanta 31527, Egypt.
Wafaa Abd ElazizDepartment of Biochemistry, Biotechnology Research Institute, National Research Centre, Dokki, Giza 12622, Egypt.
Sama MetwallyDepartment of Internal Medicine, Faculty of Medicine, Tanta University, Tanta 31527, Egypt.
Raghda Gabr MashaalDepartment of Internal Medicine, Faculty of Medicine, Tanta University, Tanta 31527, Egypt.
Rasha A Abd EllatifDepartment of Basic Dental and Medical Sciences, Faculty of Dentistry, Mutah University, Al-Karak 61710, Jordan.
Ahmed G ElkhoulyDepartment of Cardiothoracic Surgery, Faculty of Medicine, Tanta University, Tanta 31527, Egypt.ORCID 0000-0002-0491-0365
Hanan SalemDepartment of Cardiovascular Medicine, Faculty of Medicine, Tanta University, Tanta 31527, Egypt.
Amira E OraibyDepartment of Clinical Pathology, Faculty of Medicine, Tanta University, Tanta 31527, Egypt.
Bsant S KasemDepartment of Clinical Pathology, Faculty of Medicine, Tanta University, Tanta 31527, Egypt.
Sherif AbdelbakyDepartment of Tropical Medicine and Infectious Disease, Faculty of Medicine, Tanta University, Tanta 31527, Egypt.
Reham M ElkolalyDepartment of Chest, Faculty of Medicine, Tanta University, Tanta 31527, Egypt.
Marwa S TahaDepartment of Medical Microbiology and Immunology, Faculty of Medicine, Tanta University, Tanta 31527, Egypt.ORCID 0000-0002-5850-8160

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

To improve clinical decision-making about Carbapenem-resistant Gram-negative bacteria (CR-GNB) infections and halt the spread of resistant microbes, quicker and less expensive diagnostic techniques are required. Thus, the purpose of this study was to thoroughly evaluate the diagnostic efficiency (sensitivity, specificity, and concordance) of direct-from-specimen multiplex lateral flow immunoassay (LFIA) across diverse raw clinical specimens and pathogen types from critically sick patients. A total of 300 non-duplicate samples were tested to detect CR-GNB. Five major Carbapenemase genes were detected directly from the specimen using carbapenem-resistant K.N.I.V.O. detection K-Set and from culture using culture-enhanced multiplex PCR. Turnaround time (TAT) of each method was calculated. The direct LFIA revealed 100% specificity for NDM, KPC, and IMP enzymes in all tested clinical matrices (blood, urine, and respiratory samples). The study demonstrated 100% sensitivity and specificity with perfect categorical agreement (

Indexed as

Bacterial Proteinsbeta-LactamasesCarbapenem-Resistant EnterobacteriaceaeAnti-Bacterial AgentsCarbapenemsGram-Negative BacteriaHumansImmunoassayKlebsiella pneumoniaeRapid Diagnostic TestsSensitivity and SpecificityTime FactorsAnti-Bacterial AgentsBacterial Proteinsbeta-LactamasescarbapenemaseCarbapenemsbla IMPbla KPCbla NDMbla OXAbla VIMcarbapenemase genescarbapenemase-producing Enterobacterales (CRE)carbapenem-resistant Gram-negative bacteria (CR-GNB)lateral flow immunoassays (LFIA)

Identifiers

PMID42347246
PMCPMC13304714

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.