Egg Yolk Antibodies Elicited by a Novel Multi-Epitope Recombinant Adenovirus Vaccine Against Genotype G2b PEDV Spike Protein Reduce Mortality and Viral Shedding in Passively Immunized Piglets.

Cunyi Qiu, Zhiding Zhou, Meilin Yang, Huaxin Wang, Xuezhao Li, Zhihua Feng, Yefei Zhou

Abstract read

In one paragraph

Article in Pathogens (Basel, Switzerland), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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Authors and funding

7 authors.

Cunyi QiuDepartment of Food Science, Nanjing Xiaozhuang University, Nanjing 211171, China.

Zhiding ZhouCollege of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China.

Meilin YangDepartment of Food Science, Nanjing Xiaozhuang University, Nanjing 211171, China.

Huaxin WangCollege of Veterinary Science and Technology, Gansu Polytechnic College of Animal Husbandry & Engineering, Wuwei 733006, China.

Xuezhao LiCollege of Veterinary Science and Technology, Gansu Polytechnic College of Animal Husbandry & Engineering, Wuwei 733006, China.

Zhihua FengCollege of Veterinary Science and Technology, Gansu Polytechnic College of Animal Husbandry & Engineering, Wuwei 733006, China.

Yefei ZhouDepartment of Food Science, Nanjing Xiaozhuang University, Nanjing 211171, China.

Funding

2025 Innovation Fund for Young Teachers at Gansu Vocational College of Animal Husbandry Engineering GMY-QC-2025-112026 Provincial Science and Technology Program (Innovation Guidance Plan) Project of Gansu Provincial Department of Science and Technology 26CXNH001Basic Science (Natural Science) Research Project of Higher Education Institutions in Jiangsu Province 25KJA180004The 2026 Innovation Fund for University Teachers in Gansu Province 2026A-377

6 · The paper itself

Abstract

Porcine epidemic diarrhea (PED), caused by the PED virus (PEDV), remains one of the most devastating diseases in the swine industry, with a mortality rate approaching 90-100% in suckling piglets due to severe dehydration and electrolyte imbalances. Passive immunization with egg yolk antibodies (IgY) represents a promising therapeutic strategy. In this study, we developed a novel recombinant adenovirus, rADM-IFN-G-ped, co-expressing selected antigenic regions of the PEDV S protein and chicken interferon-gamma (ChIFN-γ) as a molecular adjuvant. Laying hens were immunized with this construct to produce PEDV-specific IgY, which was subsequently purified from eggs using a polyethylene glycol (PEG-6000) precipitation method. The induced IgY demonstrated potent neutralizing activity against PEDV in vitro, with a neutralization titer (NT

Indexed as

Antibodies, ViralCoronavirus InfectionsEgg YolkEpitopesPorcine epidemic diarrhea virusSpike Glycoprotein, CoronavirusSwine DiseasesViral VaccinesAdenoviridaeAnimalsAntibodies, NeutralizingChickensFemaleGenotypeImmunization, PassiveImmunoglobulinsAntibodies, NeutralizingAntibodies, ViralEpitopesIgYImmunoglobulinsSpike Glycoprotein, CoronavirusVaccines, SyntheticViral Vaccinesadenoviral vector vaccineegg yolk antibodies (IgY)pigletsporcine epidemic diarrhea virus (PEDV)

Identifiers

PMID42347214

DOI10.3390/pathogens15060602

PMCPMC13306146

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.