Evaluation of IndiMix JOE with Intype IC-RNA as an Alternative to AgPath-ID for Influenza A Virus Detection in Avian and Bovine Samples.

Andie Hach, Anne Vandenburg-Carroll, Douglas G Marthaler, Stephen Vadia, Qirui Zhang, Melanie Prarat, Ailam Lim

Abstract readEvaluation Study

In one paragraph

Article in Pathogens (Basel, Switzerland), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Andie HachWisconsin Veterinary Diagnostic Laboratory, University of Wisconsin-Madison, Madison, WI 53706, USA.

Anne Vandenburg-CarrollWisconsin Veterinary Diagnostic Laboratory, University of Wisconsin-Madison, Madison, WI 53706, USA.

Douglas G MarthalerINDICAL BIOSCIENCE, 04103 Leipzig, Germany.ORCID 0000-0001-8574-2473

Stephen VadiaOhio Department of Agriculture, Animal Disease Diagnostic Laboratory, Reynoldsburg, OH 43068, USA.

Qirui ZhangOhio Department of Agriculture, Animal Disease Diagnostic Laboratory, Reynoldsburg, OH 43068, USA.ORCID 0009-0007-9135-7230

Melanie PraratOhio Department of Agriculture, Animal Disease Diagnostic Laboratory, Reynoldsburg, OH 43068, USA.ORCID 0000-0003-2953-7541

Ailam LimWisconsin Veterinary Diagnostic Laboratory, University of Wisconsin-Madison, Madison, WI 53706, USA.ORCID 0000-0003-1546-5401

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

This method comparison demonstrated equivalent analytical and diagnostic performance of IndiMix JOE with intype IC-RNA as an alternative real-time polymerase chain reaction (RT-PCR) chemistry compared to the National Animal Health Laboratory Network (NAHLN) influenza A virus (IAV) RT-PCR surveillance assay using AgPath-ID One-Step RT-PCR Reagents in avian swabs and tissues and bovine milk. In avian samples, IndiMix JOE with intype IC-RNA using a fast reduced-volume protocol had comparable results to the NAHLN reference RT-PCR with and without intype IC-RNA using standard NAHLN thermocycling conditions. A six-way comparison of RT-PCR chemistries and exogenous internal controls in milk samples illustrated equivalent mean CT values (ANOVA,

Indexed as

Cattle DiseasesInfluenza A virusInfluenza in BirdsOrthomyxoviridae InfectionsReal-Time Polymerase Chain ReactionRNA, ViralAnimalsBirdsCattleMilkReverse Transcriptase Polymerase Chain ReactionSensitivity and SpecificityRNA, ViralAgPath-ID One-Step RT-PCR Reagentsanalytical sensitivitybovine milkbovine semendiagnostic method comparisonexogeneous internal controlshighly pathogenic avian influenzaIndiMix JOEinfluenza A virus surveillanceintype IC-RNAlimit of detectionoutbreak responsePCR inhibitionRT-PCR

Identifiers

PMID42347212

DOI10.3390/pathogens15060600

PMCPMC13305992

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.