ArticleBiosensors2026
A Centrifugal Microfluidic Platform Integrating Immunomagnetic Separation and Isothermal Amplification for Rapid and High-Sensitivity Detection of Foodborne Pathogens.
Article in Biosensors, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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6 authors.
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Abstract
The contamination by foodborne pathogens posed a significant health threat and huge economic burden. Traditional detection methods were limited by cumbersome and time-consuming procedures, low automation, and reliance on expensive instrumentation, making them inadequate for on-site detection. This paper presented a centrifugal microfluidic chip that integrated sample pretreatment, nucleic acid extraction, amplification reaction, and signal detection. The chip featured an innovative design that combined a bursting valve with the siphon channel and employed a dual-channel configuration for splitting and directing the flow of different reagents, thereby overcoming the instability issue of unintended pre-activation or interruption that often occurred in the cascade design of multilevel siphon channels. Moreover, by synergistically combining with immunomagnetic separation as well as multi-enzyme isothermal rapid amplification, a portable, easy, rapid, high-sensitivity, and low-cost point-of-care testing (POCT) system for foodborne pathogens was developed. Under optimized conditions, the system enabled detection of
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