Evidence map›Paper›PMID 42345443›Full record

ArticleEuropean journal of immunology2026

CRISPR/Cas9-Mediated Gene Knockout Reveals a Nonredundant Role for p16

Silvia Fiori, Cecilia Adragna, Emilia Malvicini, Tommaso Basini, Donatella Galgano, Edoardo Scarpa, Sandra Jovic, Niklas A Schmacke, Veit Hornung, Federica Sallusto and 3 more

Abstract read
In one paragraph

Article in European journal of immunology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Silvia FioriNational Institute of Molecular Genetics, Milan, Italy.ORCID 0009-0002-7858-4395
Cecilia AdragnaNational Institute of Molecular Genetics, Milan, Italy.ORCID 0009-0008-2924-6641
Emilia MalviciniNational Institute of Molecular Genetics, Milan, Italy.ORCID 0000-0003-2286-8954
Tommaso BasiniNational Institute of Molecular Genetics, Milan, Italy.
Donatella GalganoNational Institute of Molecular Genetics, Milan, Italy.ORCID 0000-0003-0889-0362
Edoardo ScarpaNational Institute of Molecular Genetics, Milan, Italy.ORCID 0000-0002-5886-6929
Sandra JovicInstitute for Research in Biomedicine, Bellinzona, Switzerland.
Niklas A SchmackeGene Center and Department of Biochemistry, Ludwig-Maximilians-Universität München, Munich, Germany.ORCID 0000-0002-3998-1139
Veit HornungGene Center and Department of Biochemistry, Ludwig-Maximilians-Universität München, Munich, Germany.ORCID 0000-0002-4150-194X
Federica SallustoInstitute for Research in Biomedicine, Bellinzona, Switzerland.ORCID 0000-0003-3750-2752
Ludovica BrunoNational Institute of Molecular Genetics, Milan, Italy.ORCID 0009-0006-8042-3370
Antonio LanzavecchiaNational Institute of Molecular Genetics, Milan, Italy.
Manuel AlbaneseNational Institute of Molecular Genetics, Milan, Italy.ORCID 0000-0002-4614-7599

Funding

European Research Council 885539Joachim Herz FoundationMFAG AIRC 28809
6 · The paper itself

Abstract

The possibility of enhancing T cell function by deleting specific genes represents a long-sought goal in preclinical studies and ultimately for clinical applications. Using CRISPR/Cas9 genome editing, we report that, in human cytotoxic CD8 T cell clones, the cell cycle checkpoint gene CDKN2A, encoding p16

Indexed as

CD8-Positive T-LymphocytesCRISPR-Cas SystemsCyclin-Dependent Kinase Inhibitor p16Receptors, Antigen, T-CellCell ProliferationGene Knockout TechniquesHumansLymphocyte ActivationCyclin-Dependent Kinase Inhibitor p16Receptors, Antigen, T-CellCD8 T cellscell cycleCRISPR/Cas9cytotoxicitygene editingp16INK4Aproliferation

Identifiers

PMID42345443
PMCPMC13296263

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.