Evidence map›Paper›PMID 42343256›Full record

ArticleBMC genomics2026

Identification and characterization of the HSP gene family in the Chinese giant salamander: Expression patterns under combined environmental stress.

Mei Xie, Cheng Wang, Jingfang Li, Yanhui Yin, Qilan Gao, Yixing Xie, Fangpeng Zhang, Zhiyong Deng, Ying Wei, Wansheng Jiang

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PubMed Full text at PMC Publisher
In one paragraph

Article in BMC genomics, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

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2citing papers in PubMed
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1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

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4 · The record

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PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Mei Xie *Hunan Engineering Laboratory for Giant Salamander Resource Conservation and Comprehensive Utilization, School of Life Sciences, Jishou University, Zhangjiajie, Hunan, 427000, China.
Cheng Wang *Hunan Engineering Laboratory for Giant Salamander Resource Conservation and Comprehensive Utilization, School of Life Sciences, Jishou University, Zhangjiajie, Hunan, 427000, China.
Jingfang LiHunan Engineering Laboratory for Giant Salamander Resource Conservation and Comprehensive Utilization, School of Life Sciences, Jishou University, Zhangjiajie, Hunan, 427000, China.
Yanhui YinHunan Engineering Laboratory for Giant Salamander Resource Conservation and Comprehensive Utilization, School of Life Sciences, Jishou University, Zhangjiajie, Hunan, 427000, China.
Qilan GaoZhangjiajie Institute of Aeronautical Engineering, Zhangjiajie, Hunan, 427000, China.
Yixing XieHunan Zhangjiajie Giant Salamander National Nature Reserve Affairs Center, Zhangjiajie, Hunan, 427400, China.
Fangpeng ZhangHunan Engineering Laboratory for Giant Salamander Resource Conservation and Comprehensive Utilization, School of Life Sciences, Jishou University, Zhangjiajie, Hunan, 427000, China.
Zhiyong DengHunan Zhangjiajie Giant Salamander National Nature Reserve Affairs Center, Zhangjiajie, Hunan, 427400, China.
Ying WeiHunan Zhangjiajie Giant Salamander National Nature Reserve Affairs Center, Zhangjiajie, Hunan, 427400, China. 18685100701@163.com.
Wansheng JiangHunan Engineering Laboratory for Giant Salamander Resource Conservation and Comprehensive Utilization, School of Life Sciences, Jishou University, Zhangjiajie, Hunan, 427000, China. jiangwschina@163.com.ORCID http://orcid.org/0000-0002-6498-944X

Funding

Hunan Forestry Science and Technology Research and Innovation Fund Project XLKY202303, XLKY202421Jishou University Graduate Student Research Innovation Project JDY2024067National Natural Science Foundation of China 32060128
6 · The paper itself

Abstract

backgroundThe Chinese giant salamander (Andrias davidianus) is a critically endangered living fossil species that is highly sensitive to changes in water temperature. However, systematic studies on the heat shock protein (HSP) gene family and its response mechanisms to environmental stress in this species remain limited. This study utilized transcriptome data from captive-bred salamanders exposed to combined temperature and pathogen stress. Bioinformatics tools were employed to identify the HSP gene family of A. davidianus (AndHSP) and to analyze their evolution, structure, and function, thereby revealing their regulatory mechanisms in response to environmental stress.

resultsA total of 72 AndHSPs were identified and classified into five subfamilies. Phylogenetic analysis revealed that each subfamily is evolutionarily conserved and functionally related. Gene expression analysis demonstrated that pathogen infection induced the expression of AndHSPs, and elevated temperature significantly intensified this response. Nine key differentially expressed genes were identified, predominantly from the AndHSP70 subfamily, with AndHSP70-18 exhibiting rapid heat-induced expression. Tissue-specific analysis showed high expression of AndHSP60 in the spleen. A qPCR validation confirmed the reliability of the transcriptome expression results.

conclusionsThis study presents the first systematic identification of the AndHSP gene family and elucidates its cooperative stress response mechanisms under combined temperature and pathogen stress. These findings provide a molecular basis for understanding the species' environmental adaptation and have important implications for its conservation and artificial breeding.

Indexed as

CaudataHeat-Shock ProteinsMultigene FamilyStress, PhysiologicalAnimalsGene Expression ProfilingGene Expression RegulationPhylogenyTranscriptomeHeat-Shock ProteinsChinese giant salamanderEnvironmental stressGene expressionGene familyHeat shock protein

Identifiers

PMID42343256
PMCPMC13551934

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