Evidence map›Paper›PMID 42343024›Full record

ArticleAnalytical sciences : the international journal of the Japan Society for Analytical Chemistry2026

Platinum thin film-based matrix-enhanced surface-assisted laser desorption/ionization mass spectrometry imaging for improved ion yields of adrenocortical steroids.

Riko Takata, Shigehiro Karashima, Kokoro Okawa, Akio Miyazato, Fumitake Usui-Kawanishi, Reina Nakanishi, Tatsuya Murakami, Atsushi Hashimoto, Hiroshi Kawasaki, Issey Osaka

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Analytical sciences : the international journal of the Japan Society for Analytical Chemistry, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Riko TakataDepartment of Pharmaceutical Engineering, Faculty of Engineering, Toyama Prefectural University, 5180 Kurokawa, Imizu-City, Toyama, 939-0398, Japan.
Shigehiro KarashimaInstitute of Liberal Arts and Science, Kanazawa University, Kanazawa, Ishikawa, 920-1192, Japan. skarashima@staff.kanazawa-u.ac.jp.
Kokoro OkawaDepartment of Pharmaceutical Engineering, Faculty of Engineering, Toyama Prefectural University, 5180 Kurokawa, Imizu-City, Toyama, 939-0398, Japan.
Akio MiyazatoCenter for Nano Materials and Technology, Japan Advanced Institute of Science and Technology, Nomi, Ishikawa, 923-1292, Japan.
Fumitake Usui-KawanishiDepartment of Pharmaceutical Engineering, Faculty of Engineering, Toyama Prefectural University, 5180 Kurokawa, Imizu-City, Toyama, 939-0398, Japan.
Reina NakanishiDepartment of Pharmaceutical Engineering, Faculty of Engineering, Toyama Prefectural University, 5180 Kurokawa, Imizu-City, Toyama, 939-0398, Japan.
Tatsuya MurakamiDepartment of Pharmaceutical Engineering, Faculty of Engineering, Toyama Prefectural University, 5180 Kurokawa, Imizu-City, Toyama, 939-0398, Japan.
Atsushi HashimotoFaculty of Transdisciplinary Sciences, Institute of Philosophy in Interdisciplinary Sciences, Kanazawa University, Kanazawa, Ishikawa, 920-1192, Japan.
Hiroshi KawasakiDepartment of Medical Neuroscience, Graduate School of Medical Sciences, Kanazawa University, Kanazawa, Ishikawa, 920-8640, Japan.
Issey OsakaDepartment of Pharmaceutical Engineering, Faculty of Engineering, Toyama Prefectural University, 5180 Kurokawa, Imizu-City, Toyama, 939-0398, Japan. o-issey@pu-toyama.ac.jp.ORCID http://orcid.org/0000-0002-3642-2278

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Matrix-assisted laser desorption/ionization mass spectrometry (MALDI/MS) imaging enables spatially resolved molecular analysis; however, its sensitivity depends strongly on matrix uniformity and ionization efficiency, which pose challenges for low-abundance compounds such as steroid hormones. In this study, we developed a platinum (Pt) thin film-based surface-assisted laser desorption/ionization mass spectrometry (SALDI/MS) and matrix-enhanced SALDI/MS (ME-SALDI/MS) method to increase ion yields and improve steroid imaging with minimal sample pretreatment. Using sputter-deposited Pt films, 20 endogenous steroids were comprehensively detected without chemical derivatization. Furthermore, derivatized steroids analyzed by ME-SALDI/MS exhibited significantly higher ion yields than those obtained by conventional MALDI/MS or SALDI/MS, demonstrating a synergistic enhancement of desorption and ionization efficiency. ME-SALDI/MS imaging of porcine adrenal gland tissue enabled visualization of multiple derivatized steroids with high ion yields-including progesterone, corticosterone, and cortisol-reflecting their biosynthetic localization across cortical zones. Collectively, these results highlight the potential of Pt thin film-based SALDI and ME-SALDI/MS as robust tools that provide high ion yields for imaging of steroids and other biomolecules with inherently low desorption and ionization efficiency.

Indexed as

Adrenal CortexPlatinumSpectrometry, Mass, Matrix-Assisted Laser Desorption-IonizationSteroidsAnimalsIonsSurface PropertiesSwineIonsPlatinumSteroidsAdrenocortical steroidsMALDIME-SALDIMS imagingSALDI

Identifiers

PMID42343024
PMCPMC13493469

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.