ArticleInternational dental journal2026
BMP9-Smad1/5/9 Signalling Mediates Osteogenic Differentiation and Alveolar Bone Remodelling in hPDLFs.
Article in International dental journal, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
objectivesThis study aimed to investigate the role of BMP9-Smad1/5/9 in the osteogenic differentiation and alveolar bone remodelling of Human periodontal ligament fibroblasts (hPDLFs) under the action of cyclic tensile stress (CTS).
methodsAn orthodontic tooth movement (OTM) model was established in Sprague-Dawley (SD) rats, the expression status of OCN and BMP9 was measured using immunohistochemistry. hPDLFs were subjected to CTS treatment (10% elongation, 0.5 Hz) to simulate the mechanical microenvironment during orthodontic treatment. The activity of ALP was detected by colorimetric assay and azo coupling staining, respectively. Mineralized nodules were visualized by Alizarin red staining. The expression levels of osteogenesis-related genes including RUNX2, ALP, and OCN were evaluated. To determine the role of BMP9-Smad1/5/9 in CTS-induced osteogenic differentiation, BMP9-Smad1/5/9 signalling was diminished by small RNA against BMP9 and the inhibitor of Smad signalling, the potential of osteogenic differentiation was assessed.
resultsBMP9 expression was upregulated in the tension-side periodontal ligament tissues of SD rats, as well as in hPDLFs stimulated by CTS. hPDLFs exhibited increased osteogenic differentiation potential under CTS induction, as evidenced by increased ALP activity, upregulated expression of osteogenesis-related genes, increased mineralized nodule formation and ALP staining. Mechanistically, CTS elevated the phosphorylation levels of Smad1/5/9. Silencing BMP9 attenuated CTS-induced osteogenic differentiation and reduced Smad1/5/9 phosphorylation. Moreover, inhibition of BMP9-Smad1/5/9 signalling pathway suppressed the osteogenic potential of hPDLFs.
conclusionsCTS increased the osteogenic potential of hPDLFs. Silencing of BMP9 and inhibition of the BMP9-Smad1/5/9 signalling inhibited the osteogenic differentiation of hPDLFs induced by CTS. CLINICAL RELEVANCE: These findings provide mechanistic insights into periodontal tissue remodelling and may contribute to the development of strategies to accelerate orthodontic tooth movement in clinical practice.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.