ArticleApplied microbiology and biotechnology2026
Glutathione peroxidase gene regulates heat stress response via trehalose synthesis in Pleurotus ostreatus.
Article in Applied microbiology and biotechnology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors.
Funding
Abstract
Heat stress (HS) is a critical bottleneck restricting the commercial cultivation of Pleurotus ostreatus, severely compromising its yield and quality. Glutathione peroxidase (GPX) is a conserved antioxidant enzyme essential for stress adaptation, yet its regulatory mechanism in the fungal heat stress response (HSR) remains unclear. This study characterized a GPX homolog PoGPX in P. ostreatus and elucidated its functions in fungal growth, abiotic stress resistance, and HSR. PoGPX was downregulated during fruiting body development and upregulated under multiple abiotic stresses (especially HS). Silencing PoGPX promoted mycelial growth, biomass accumulation, and hyphal hyperbranching, accompanied by elevated intracellular reactive oxygen species (ROS) levels and compensatory changes in antioxidant enzyme activities. Notably, silencing PoGPX increased cytosolic trehalose content by 0.45-0.62-fold compared with the wild type (WT) strain, and this elevation was reversed by treatment with the ROS scavenger N-acetylcysteine, implying PoGPX modulates trehalose biosynthesis through ROS signaling. Functionally, silencing PoGPX alleviated HS-induced oxidative damage (reduced malondialdehyde content) and suppressed the upregulation of heat shock protein (HSP) genes expression (HSP60, HSP90, HSP104). Critically, inhibiting trehalose biosynthesis restored the HSR phenotypes in PoGPX-silenced strains, indicating trehalose as the key downstream mediator of PoGPX-regulated HSR. These findings uncover that PoGPX regulates HSR in P. ostreatus through trehalose biosynthesis induced by intracellular ROS levels. This study provides novel insights into fungal HSR mechanisms and identifies PoGPX as a promising target for genetic engineering to improve the thermotolerance of P. ostreatus in commercial cultivation. KEY POINTS: • PoGPX acts as a key negative regulator on mycelial growth and hyphal branching in P. ostreatus. • The PoGPX silencing alleviates the heat stress response of P. ostreatus. • PoGPX regulates the heat stress response in P. ostreatus by modulating cytosolic trehalose biosynthesis.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.