Evidence map›Paper›PMID 42340399›Full record

ArticleMicrobial ecology2026

Metagenomic Analysis of Rural Groundwater Viromes Reveals Bacteriophage Contributions to Groundwater Microbial Ecology.

Manar-Aleslam M Mattar, Walaa A Eraqi, Mohamed Bakr Zaki, Akram M Elkashlan, Khaled A M Abouzid, Ramy K Aziz, Aymen S Yassin, Ali H A Elbehery

Abstract read
In one paragraph

Article in Microbial ecology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Manar-Aleslam M MattarDepartment of Microbiology and Immunology, Faculty of Pharmacy, University of Sadat City, Sadat City, Egypt.
Walaa A EraqiDepartment of Microbiology and Immunology, Faculty of Pharmacy, Cairo University, Cairo, Egypt.ORCID http://orcid.org/0000-0003-0798-9125
Mohamed Bakr ZakiDepartment of Biochemistry, Faculty of Pharmacy, University of Sadat City, Sadat City, Egypt.
Akram M ElkashlanDepartment of Biochemistry, Faculty of Pharmacy, University of Sadat City, Sadat City, Egypt.
Khaled A M AbouzidDepartment of Pharmaceutical Chemistry, Faculty of Pharmacy, Ain Shams University, Cairo, Egypt.
Ramy K AzizDepartment of Microbiology and Immunology, Faculty of Pharmacy, Cairo University, Cairo, Egypt.ORCID http://orcid.org/0000-0002-4448-7100
Aymen S YassinDepartment of Microbiology and Immunology, Faculty of Pharmacy, Cairo University, Cairo, Egypt. aymen.yassin@pharma.cu.edu.eg.ORCID http://orcid.org/0000-0002-4423-164X
Ali H A ElbeheryDepartment of Microbiology and Immunology, Faculty of Pharmacy, University of Sadat City, Sadat City, Egypt. ali.elbehery@fop.usc.edu.eg.ORCID https://orcid.org/0000-0002-8028-9849

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Groundwater ecosystems host diverse microbial communities, yet the diversity and ecological roles of their associated viral genomes remain poorly characterized. Here, we investigated viral community composition, diversity, host associations, lifestyles, and auxiliary metabolic potential in groundwater from three hand pumps located in Toukh, Qalyubia, Egypt, representing distinct local surroundings and potential contamination pressures. Using complementary viral detection approaches and a quality assessment workflow, we recovered 9,534 non-redundant viral contigs spanning a wide range of viral genome quality. Taxonomic profiling revealed dominance of tailed dsDNA bacteriophages (Uroviricota/Caudoviricetes) across all pumps, with ~ 99% of contigs not assigned below the class level. Whereas the viral composition of pump 3 was distinct and its diversity was consistently higher, pumps 1 and 2 clustered together, a pattern mirrored across taxonomic scales and diversity metrics. The majority of predicted viral hosts belonged to phylum Pseudomonadota, followed by Actinomycetota, Bacillota and Bacteroidota, with levels that varied between pumps. Correlation and network analyses showed strong concordance between the relative abundance of bacteria and the abundance of viruses that potentially infect them. Lifestyle prediction indicated a descending relative abundance of viruses with lysogenic lifestyle from pumps 1 through 3. Auxiliary metabolic genes (AMGs) related mainly to nucleotide, amino acid, and cofactor metabolism were detected in all pumps, with distinct pump-specific repertoires suggesting localized viral metabolic strategies. Together, these results demonstrate that groundwater viromes are ecologically structured and highly novel, with the potential ability to modulate host metabolism, highlighting their potential role in shaping subsurface microbial communities.

Indexed as

BacteriaBacteriophagesGroundwaterViromeEcosystemGenome, ViralMetagenomicsMicrobiotaPhylogenyAuxiliary metabolic genesBacteriophagesEnvironmental metagenomicsGroundwater viromeViral ecologyVirus–host interactions

Identifiers

PMID42340399
PMCPMC13303688

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.